The Cili Polysaccharide Separation And Purification, Physicochemical Properties And Biological Activity

In this paper,polysaccharides were extracted and isolated from Rosa roxburghii Tratt juice,and their physicochemical characteristics and bioactivities were studied.The main contents are as follows:1.Isolation,purification and physicochemical characteristics of Rosa roxburghii polysaccharides.RRTP-1～RRTP-8 were obtained by concentration,precipitation with ethanol, deprotein and decoloufing,DEAE-cellulose column and Sepharose CL-6B column chromatography from the Rosa roxburghii Tratt juice.RRTP-1 and RRTP-3 were confirmed to be homogeneous composition by gel chromatography and polyacrylamide gel electrophoresis,and the IR spectrum showed typical characteristic absorption peak of polysaccharides.The molecular weight of RRTP-1 and RRTP-3 were measured to be 3200 and 52000 respectively by gel permeation chromatography.By GC analysis, RRTP-1 was consisted of rhamnose,arabinose,unknown sugar,xylose,mannose, galactose,the molar ratio of neutromonosaccharide were 1.00:16.75:13.37:5.86:11.49: 22.73:7.80;RRTP-3 was consisted of rhamnose,arabinose,unknown sugar,mannose, galactose,glucose,with the molar ratio of neutromonosaccharide 1.00:5.47:1.31:3.02: 15.88:1.58.The uronic acid content of RRTP-1 and RRTP-3 were 12.7%and 16% respectively.2.The bioactivities of Rosa roxburghii Tratt polysaccharidesCrude and pure polysaccharides had different scavenging effect on hydroxy free radical,superoxide anion free radical and DPPH free radical,and they had dose-effect relationship.Forthemore,the scavenging effects of the crude polysaccharide on the three kinds of free radical were better than those of pure fraction on the same concentration.Middle and high dosage of polysaccharides can increase the activity of SOD,CAT in plasma,liver and brain in aged mice significantly,while the content of MDA were decreased,and it had dose-effect relationship.It suggested that Rosa roxburghii Tratt polysaccharides can enhance the antioxide capability of aged mice.The neural stem cells damage of test group adding RRTP-1 were alleviated,the cells death rate and LDH leakage of high concentration test group were significantly lower than those of Na₂S₂O₃ and glutamic acid group.It suggested that RRTP-1 has protective effect on neural stem cells damage caused by Na₂S₂O₃ and glutamic acid.Most of polysaccharide fractions can induce differentiation of PC12 cell and had neural nutrition activity.While in our dosage range,pure polysaccharide fractions RRTP-1 and RRTP-3 show no dose-effect relationship on positive rates of PC12 cell.