Preparation Of Fluorescent Magnetic Nanoparticles And Their Applications In Magnetic Resonance

The rapid progress of nanoscience and nanotechnology in past decade has made itself being widely applied into many other subjects, and caused the formation of a series of independent but close related interdisciplinaries. Bionanomedicine, which is composed by nanoscience, biology, medicine, is one of the most shining and active research field. One major content of it is the preparation and application of functional biomedical materials. The luminescent silica dioxide , which has a superior fluorescence nanometer effect, was widely applied in research and preparation of fluorescence mark probe and fluorescence sensors. Magnetic nanomaterials, which have advantages of the small size and the strong controllability, are applied successfully in disease's diagnosis and treatment as well as biological materials' separation, particularly it has the very good application prospect as the contrast agent in the magnetic resonance image. This article is focused on the preparation of the luminescent silica dioxide and the magnetic nanomaterials, and has carried on the preliminary discussion on magnetic nanomaterials as the contrast agent used in the magnetic resonance imaging..(1) This article provides a 'one-pot' process for growing luminescent silica dioxide spheres under basic conditions without the subsequent addition of inorganic or organic fluorophores. This article also provides the configuration of silica dioxide spheres by TEM and SEM. It also illustrates fluorescence intension by fluorescence spectrometer, and it also researches the effect of calcinations temperature to fluorescence intensity of silica dioxide spheres. The result indicates that: the silica dioxide nanoparticles is spherical and uniform in size , about 160nm in diameter; In addition of organic silane in processes and calcinations often make the silica dioxide spheres exhibit luminescence, the fluorescence emission wavelength is about 380nm at all times. As the calcinations temperature changes, the fluorescence intensity cuts after increasing first, and the fluorescent intensity maximum appears in the calcinations temperature of 500℃. (2) The magnetic nanoparticles were synthesized by the chemical coprecipitation. And the magnetic nanoparticles were coated with macromolecules in the microemulsion. The surface of the nanoparticles was conjugated with a cancer-targeting antibody. Then the functional magnetic nanoparticles were used to detect pancreatic tumor cells. The results showed that the functional magnetic nanoparticles may effectively target to pancreas cancer cells, which offered a new tool for original diagnose of pancreas caner in cell lever.(3) Based on the success of the functional magnetic nanoparticles used in diagnose of pancreas cells in cell lever, we fabricated the biofunctional magnetic nanoparticles contrast agents and established the pancreas cell line BXPC-3 bare mouse hypodermic transplant lump model for magnetic resonance image research. MRI experiments results in vivo indicated the synthesized conjugated nanoparticles can successfully target to the pancreas cancer and enhance contrast of the tumor and the normal tissue in the magnetic resonance image. Therefore, these target-specific magnetic nanoparticles have potential as contrast agents use in MR imaging of pancreas cancer in vivo.