Monosaccharide, Disaccharide Sugars By High Performance Liquid Chromatography Chiral Stationary Phase Preparation And Applications

The main works of the thesis are as follows:A review of chiral separation is given.Since saccharide is a kind of natural chiral compound, four new chiral columns from monosaccharides were prepared. Some racemates were separated on these chiral stationary phases using hexane/isopropanol (90:10, v/v) as mobile phase by high-performance liquid chromatography. Among these chiral stationary phases, D-glucose chiral stationary phase gave the best enantioseparation.Enantioseparations using cellobiose and lactose as chiral selector bonded to silica gel via an arm in HPLC were reported. Surprisingly, D-cellobiose also possessed high enantioselectivity, may be used in normal-phase and reversed-phase mode.D-raffinose chiral stationary phase was prepared by two synthetic routes. With hexane/isopropanol (90:10, v/v) and methanol/water (50:50, v/v) as mobile phase in HPLC, D-raffinose chiral column exhibited a good separation for some chiral compounds. This study indicates that oligosaccharides could soon become very attractive as a new class of chiral stationary phase for HPLC.