The Role Of Cdte Molecules Fluorescently Labeled Biomolecules

This article synthesize four different quantum dots, evaluate the fluorescence capability respectively. CdTe QDs were labeling with albumen, crank out fluorescence probe.Then evaluate the stabilization and toxicity of QDs in gel column.This devide into three parts:1 Water-soluble CdTe quantum dots were prepared in aqueous solution by pressure pan and reflux,with using mercaptopropanoic acid as the stabilizer. Different emission wavelengths of quantum dots(QDs)were synthesized by changing the nucleation time, and then characterized by fluorescence spectroscopy, UV-Visible spectroscopy, and ICP-MS, respectively. The results show that these quantum dots have good optical properties, such as narrow spectral line widths and continuous absorption profiles, which provide a powerful foundation for further applications. These quantum dots possess narrow and symmetrical fluorescent emissions. The emission wavelengths of these composite dots span most of the visible spectrum from 500 nm through 700 nm. In our study, CdTe QDs were labeling with BAS and OVA by covalence coupled action, and it is confirmed that QDS-BAS and QDS-OVA complexes had good fluorescent properties, and this study supplied solid foundation for labeling protein in the future reseacher.2 Water-soluble CdTe, CdTe/CdS, CdTe/CdS/ZnSquantum dots were prepared in aqueous solution by pressure pan, with using mercaptopropanoic acid as the stabilizer. The synthesized QDs were characterized by UV-vis fluorescence spectrophotometer and gel chromatography. Evaluate the stabilization of QDs in gel column, with different length of gel column and different mobile phase.3 With mercaptopropanoic acid modified water-soluble CdTe quantum dots (QDs), CdTe/SiO2 QDs was synthesized by using a quaternary "water-in-oil" microemulsion system method. The synthesized QDs were characterized by UV-Visible spectroscopy and Fluorescence Spectrophotometer. The extent of coating and the elemental ratio of CdTe and CdTe/SiO2 QDs were measured by inductively coupled mass spectrometer (ICP-MS). Based on the dialysis method, ion releasing behavior of CdTe and CdTe/SiO2 QDs were studied. Then an alga, Phaeodactylum tricornutum, was cultured with the same total cadmium concentration of the these QDs to evaluate their cytotoxicty discrepancy for the first time. These results exhibit that the cytotoxicty of QDs in organism is influenced by shell. The cytotoxicity of QDs to the Phaeodactylum tricornutum decreased with the shell growth of SiO2.