Lactobacillus Plantarum Lowering Cholesterol Research

It was showed that cerebrovascular and heart disease, such as artherosclerosis(AS),coronary artery disease(CAD),cerebral apoplexy were more universal and was first killer for human health. So there has been an upsurge in finding ways to lower plasma cholesterol levels in human. Several studies have indicated the cholesterol-reducing ability of Lactobacillus acidophilus. However, the rules for screening of cholesterol-lowering strains and its mechanisms were not very clear. This paper could be divided into three parts:It was found that all of the four Lactobacillus acidophilus holding in the laboratory had strong ability of pH tolerance. Lactobacillus plantarum had strong ability of bile-salt, artifice gastric juice and artificial intestinal juice tolerance too. And the adherent rate to colon adenocarcinoma cell line HT-29 of Lactobacillus plantarum was 3.11±0.52.The effects of Lactobacillus plantarum on blood fat were investigated in artificially-induced hyperlipemial mice. The results showed that after 17 day's animal experiment, the concentration of TC of rats decreased significantly, The concentration of TG and LDL-C lowed too. Mean while, a decrease in Al and an increase in HDL-C/TC value were observed. The result indicated that Lactobacillus plantarum had effect on cholesterol and blood fat reduction.In the studying of the mechanism of the cholesterol-reducing ability of Lactobacillus plantarum, it was found that the strain could assimilate and precipitate cholesterol during growth, and more cholesterol reduced was due to its cholesterol assimilating ability. Among the removal rate (24.95%), the rate of assimilation accounted for 18.83%. It was also found that the strain differed significantly in the amount of cholesterol reducing during growth with different concentration of bile salts. The result showed that the strain had the highest removal effectiveness when the concentration of bile salts in the medium was 0.4%. Cells grown in the presence of cholesterol were more resistant to lysis by sonication than were those grown in their absence, Suggesting that the strain had the ability of assimilating cholesterol.