The Effect Of Cisplatin On The Expression Of Genes Involved In Dna Damage Response In Human Lung Cancer Cell Line Ltep-a-2

ObjectiveTo investigate the effect of cisplatin on the expression of genes involved in DNA damage response in human lung cancer cell line LTEP-a-2. Methods1. The effect of cisplatin on the growth of human lung caner cell line LTEP-a-2 was studied with CCK-8 assay.2. The effect of cisplatin on the cell cycle phase distribution and apoptosis in LTEP-a-2 cells was determined using flow cytometry by a propidium iodide method.3. The effect of cisplatin on the expression of genes involved in DNA damage response in human lung cancer cell line LTEP-a-2 was studied with PCR assay.Results1. Cisplatin inhibited the proliferation of LTEP-a-2 cells.2. Flow cytometric analyses showed that LTEP-a-2 cells treated with cisplatin were accumulated in G2/M phase in cell cycle.3. The expression of genes involved in DNA damage response changed in LTEP-a-2 cells treated with cisplatin,The expressions of PCBP4 and TP73 were upregulated, while DDB1 and LIG1 were downregulated. ConclusionThe expressions of genes involved in DNA damage response changed in LTEP-a-2 cells treated with cisplatin. The effect of cisplatin on the expression of genes involved in DNA damage response may be corrected with the mechanism of action of cisplatin.