| Objective:1.To investigate the relationship between bone mass (bone mass content, BMC, bone mass density, BMD) and body mass index (BMI)-related obesity in Chinese Hans lives in Jinan.2.To investigate the relationship between bone mass (bone mass content, BMC, bone mass density, BMD) and waistline (WL)-related obesity in Chinese Hans lives in Jinan.3. To investigate the relationship between bone mass (bone mass content, BMC, bone mass density, BMD) and the skin fold thickness of anterior superior iliac spine and abdomen in Chinese Hans lives in Jinan.4.To investigate the relationship between bone mass (bone mass content, BMC, bone mass density, BMD) and the avoirdupois, the quantity of fat and muscles at every pore in Chinese Hans lives in Jinan.5. In this study we aimed at investigating the association of rs 1884052 and rs3778099, and another polymorphism (rs2234693) located at intron 1 of the ER-alpha gene with BMD, body mass index (BMI), glucose, triglyceride, and total cholesterol (CHO) levels in Chinese Hans.Methods: 1.Overall,287 consecutively subjects were recruited in Jinan Maternity And Child Care Hospital from healthy volunteer. BMC and BMD of all subjects were measured by dual energy X-ray absorptiometry. Body weight, height was measure to calculate BMI ((the ratio of weight to square of height in kilograms per square meter) Overweight was defined as a BMI greater than or equal to 24 kg/m2, obesity was defined as a BMI greater than or equal to 28kg/m2. Analysis of covariance (ACNOVA) was used to evaluate differences in bone mass according to BMI- related obesity after account for confounders age and gender. Co-related factor analysis (BMC and BMD) of BMI was analyzed with partial correlation.2.Overall,265 consecutively subjects were recruited in Jinan Maternity And Child Care Hospital from healthy volunteer. BMC and BMD of all subjects were measured by dual energy X-ray absorptiometry. WL was measure. Obesity was defined as a WL greater than or equal to 85cm of man and 80cm of woman.Analysis of covariance (ACNOVA) was used to evaluate differences in bone mass according to WL- related obesity after account for confounders age and gender. Co-related factor analysis (BMC and BMD) of WL was analyzed with partial correlation.3.Overall,287 consecutively subjects were recruited in Jinan Maternity And Child Care Hospital from healthy volunteer. BMC and BMD of all subjects were measured by dual energy X-ray absorptiometry. the skin fold thickness of anterior superior iliac spine and abdomen were measure. Co-related factor analysis (BMC and BMD) of the skin fold thickness(anterior superior iliac spine4.Overall,287 consecutively subjects were recruited in Jinan Maternity And Child Care Hospital from healthy volunteer. BMC and BMD of all subjects were measured by dual energy X-ray absorptiometry. The avoirdupois and the quantity of fat and muscles at every pore were measure and those were analyzed with partial correlation. and abdomen) were analyzed with partial correlation.5.We recruited 425 consecutive adult volunteers who had a physical examination in the Jinan Maternity and Child Care Hospital. We did not observe significant association of rs1884052 and rs3778099 with BMD, BMI, glucose, triglyceride, and total cholesterol (CHO) levels.Results1. Partial analysis showed that there is a linearity positive correlation between bone mass and BMI. After controlling for gender and age, correlation coefficients of BMI with BMC in all over the body, skull, extremitas superior, extremitas inferior, pars trunci and backbone of Peason were 0.477,0.304,0.410,0.427,0.546 and 0.468, (P<0.0001). Correlation coefficients of BMI with BMD in all over the body, skull, extremitas superior, extremitas inferior, pars trunci and backbone of Peason were 0.426,0.261,0.340,0.472,0.408 and 0.370, (P<0.0001). ACNOVA showed that there is a significant difference between bone mass and BMI-related obesity after adjusted for age and gender. The BMD of obesity group were 1.190±0.016, 2.25±0.34,0.88±0.09,1.26±0.11,1.02±0.10,1.16±0.14, respectively, significant higher than those of overweight group and normal weight group. The BMC of obesity group were 2099.70±700.78,519.96±77.39,365.52±89.83,1012.28±173.79, 872.21±181.18,257.49±50.41, respectively, significant higher than those of overweight group and normal weight group.2.Partial analysis showed that there is a linearity positive correlation between bone mass and WL. After controlling for gender and age, correlation coefficients of WL with BMD in all over the body, extremitas inferior, pars trunci, cervical rib, basin and backbone of Peason were 0.296,0.306,0.340,0.368,0.381 and 0.260 (P<0.01). Correlation coefficients of WL with BMC in all over the body, extremitas inferior, pars trunci, cervical rib, basin and backbone were 0.337,0.391,0.327,0.358,0.302 and 0.269 (P<0.01). ACNOVA showed that there is a significant difference between bone mass and WL-related obesity after adjusted for age and gender. The BMD of obesity group were 1.14±0.11,1.20±0.15,0.95±0.11,0.73±0.08,1.13±0.15, 1.09±0.15, respectively, significant higher than those of control group. The BMC of obesity group were 2547.83±531.04,913.82±212.63,812.71±198.31,256.04±71.75, 315.01±82.35,241.48±53.15, respectively, significant higher than those of control group.3.Partial analysis showed that there is a linearity positive correlation between bone mass and the skin fold thickness (anterior superior iliac spine and abdomen). After controlling for gender and age, correlation coefficients of the skin fold thickness(anterior superior iliac spine and abdomen) with BMD and BMC in all over the body, extremitas superior, extremitas inferior, pars trunci, ribs, the pelvis and backbone of Peason were 0.281 and 0.235,0.175 and 0.150,0.182 and 0.137,0.245 and 0.198,0.326 and 0.269,0.299 and 0.261,0.296 and 0.247,0.322 and 0.279, 0.324 and 0.267,0.341 and 0.293,0.290 and 0.235,0.220 and 0.194,0.245 and 0.221 (P<0.05).4. After controlling for gender and age, there is a positive relationship between the avoirdupois with BMD and BMC in all over the body (P=0.0000).The extremitas superior, extremitas inferior, pars trunci, the pelvis and backbone of Peason were 0.751,0.659,0.690,0.326 and 0.269,0.299 and 0.261,0.296 and 0.247,0.322 and 0.279,0.324 and 0.267,0.341 and 0.293,0.290 and 0.235,0.220 and 0.194,0.245 and 0.221 There is a positive relationship between the correlation the quantity of fat and muscles with BMD and BMC in all over the body (P=0.0000),especially the quantity of muscles.The numerical value of r was between the 0.271 to 0.905.5.For rs2234693, increased levels of BMD for hip, spine or whole-body regions were consistently observed in TT/TC genotype carriers than in CC genotype carriers, although the board line significance diminished after adjusting for age and gender. However, significant association of rs2234693 with glucose and CHO levels were observed in our sample. Subjects with TC/CC genotypes were associated with an increased level of glucose (p=0.013) and CHO (p=0.032) levels than subjects with TT genotypes.Conclusions:1.There is a positive relationship between obesity and bone mass in Chinese Hans.2.T here is a positive relationship between obesity and bone mass in Chinese Hans.3. There is a positive relationship between the skin fold thickness(anterior superior iliac spine and abdomen) and bone mass(BMC and BMD) in Chinese Hans.4.There is a positive relationship between the bone mass(BMC and BMD) at every pore and the quantity of fat and muscles in the same position, avoirdupois in Chinese Hans.5..In conclusion, we did not confirm the association of rs1884052 and rs3778099 with BMD originally discovered in a GWA study; however, we made novel discoveries that rs2234693 was associated with glucose and CHO levels in Chinese Hans. |
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