The Research Of Radiation Damage Repairemechanism Of Bushentianjing Fa To Mice Bone Marrow Way In Atm/atr Pathway

Radiation hazards to humans more and more attention, although radiation can cause immune, blood, reproductive and other multi-system damage, but the fundamental reason leading to this important radiation target DNA damage, From the outside are fever perspective study invigorating the kidney and fill in a law to regulate radiation damage to the role of the mechanism.Aims:Observed Bushentianjing side Co60y ray radiation injury in mice induced by the role model, and by looking at its DNA damage repair ATM/ATR pathway, anti-radiation of the party mechanism.Methods:The male and female half and half balb/c mice were divided into normal group, pathological control group, kidney group, blood group and yin group. Ray irradiated mice with Co60y model after model in the bone marrow using immunohistochemical methods on key genes ATM/ATR signaling pathway related protein and image analysis were analyzed; the removal of animal eye peripheral blood, their blood for blood analysis; testis after modeling, and ovary, using HE staining to observe pathological.Results:1. Immunohistochemical results:kidney group and blood group compared with the pathological aspects of the ATR protein expression differences (P<0.05) while the ATM gene in each group were not significantly different.2. HE staining of the reproductive system:testes extent of the damage kidney group compared with model group, the difference is larger, more obvious repair; blood and yin group degree of impairment less severe compared with model group; ovarian kidney group, blood group and the yin group to retain most of the outer granular cells, that some inner necrosis, shedding some of oocyte damage, extent of damage than those in model group.3. Peripheral Results:WBC, neutrophil, Kidney group and blood group and pathological group differences (P<0.05), lymphocytes of view, yin difference between group and pathological group (P<0.05) and hemoglobin of view, three groups and pathology group had significant difference (P<0.05).Conclusion:1. Bushentianjing be for ATR gene expression; 2. Bushentianjing, to a certain extent, the reproductive function of irradiated mice has a protective effect; 3. Bushentianjing side for bone marrow after radiation Cells have repair certain promotion, its more obvious for white blood cell proliferation. Summing Bushentianjing side with anti-radiation, protecting reproductive function in mice, and promote the growth of blood cells.