Human Papillomaviruses Infections In Women With Clinical Gynecologic Diseases In Chongqing

BackgroundHuman papillomaviruses (HPVs) are DNA viruses with a genome size of about 8000 base-pairs. There are more than 100 HPV types defined on the basis of DNA homology, of which more than 40 infect the anogenital tract. Genital HPV types are typically divided into two groups according to their presumed oncogenic potential. HPV types 16,18,31,33,35,39,45, 51,52,56,58,59 and 68 are considered to be of high oncogenic risk. The remaining genital types—types 6,11,42,43 and44—are considered of low or no oncogenic risk. Subclinical and clinical genital warts, also known as condylomata acuminata, and virtually all squamous cell cancers of the anogenital tract are caused by specific HPV types. More than 20 types of human papillomavirus, especially HPV type 16 and 18, have been associated with cervical cancer, the second cause of cancer related mortality in women globally. The advent of molecular biology tools in HPV diagnosis has allowed us to identify HPV infection, differentiate HPV types, and discriminate groups of the population with different risks of infection.Objective1. To obtain the epidemiological data of Human papillomaviruses infection among women with different ages and gynecologic disease.2. To evaluate the clinical role of reverse line blot (RLB) in detecting and genotyping HPV.Methods1. Specimens (normal group and abnormal group) from women who wanted to do HPV detection were detected with the assay of reverse dot blot. The data was analyzed with the statistical software SPSS 13.0.2. With the method of RLB and multi-analyte suspension array (MASA),50 random specimen were detected for HPV infections. Then the results were analyzed.ResultsFor the normal group,19.5%(98/502) specimens were detected positive for HPV infection.2268 women were included in the abnormal group and 795(35.1%) specimens were detected positive for HPV DNA, including 6177(77.6%) one-type infection,123(15.7%) diplex infection and 55(6.9%) multiple infection. Of 795 positive specimen,558(70.2%) were high-risk HPV infection, including 235(46.1%) HPV 16 type. The difference between the HPV infection of normal group and abnormal group was significant(P<0.01). And there was significant difference of HPV infection among women with different ages, sexual history, gynecologic diseases and the ages of first sex behavior.Conclusions1. In the screening of HPV infection, we can select the high-risk population so as to decrease the cost of screening.2. Amang the 23 HPV types, HPV 16 type was the most prevalent, which suggested that HPV 16 type played an important role in the progression of cervical diseases and we should pay a lot attention to the research of the virus.3. RLB plays an important role in detecting and genotyping clinical HPV.