1,effect Of Total Saponins Of Panax Ginseng On Expression Of Signal Transducer And Activator Of Transcription 3 In K562 Cells 2,study On Artesunate Water Extract In A549 And Mcf-7 Cell Lines In Vitro

Objective:To study the expression and distriction of signal transducer and activator of transcription 3 (STAT3) in K562 cells induced by total saponins of panax ginseng(TSPG).Methods : K562 cells were divided into two groups: Control and TSPG. The TSPG group were subdivided into 5 subgroups according to the time of K562 cells induced by TSPG while the concentration of TSPG was 200μg/m in each subgroups. The TSPG group were induced for 6,12,24,36,48 hours .The expression and distriction of STAT3 in the cytoplasm and nucleus of K562 cells both in the control and TSPG groups was detected by ELISA assay , and 12 hours and 36 hours groups among them were examined for STAT3 by Immunocytochemical staining and the results were undergone an image analysis for average optical. The expression and distriction of STAT3 in the cytoplasm and nucleus of K562 cells both in the control and TSPG groups was tested by western blotting assay. 12 hours and 36 hours groups of TSPG were observed for STAT3 by laser Confocal.Results: The expression and distriction of STAT3 in the TSPG groups except 36 hours group had significant time-dependent relation(p<0.01) and significant deviation between the control group and the TSPG groups(p<0.01): Absorbance value of STAT3 in the cytoplasm were 0.350±0.040 in the control group, 0.306±0.038 when induced by TSPG for 6h,and that was down to 0.170±0.037 when induced by TSPG for 12h, 0.182±0.032 for 24h , 0.576±0.148 for 48h; Absorbance value of STAT3 in the nucleus were 0.536±0.224 in the control group, 0.597±0.247 when induced by TSPG for 6h, and that was up to 0.762±0.249 when induced by TSPG for 12h, 0.716±0.218 for 24h, 0.576±0.148 for 48h. Immunocytochemical staining showed the expression and distriction of STAT3 decreased in the cytoplasm, increased in the nucleus when induced by TSPG for 12h, while no significant deviation between the the control group and 36 hours group(P>0.05). Western blotting analysis showed the expression and distriction of STAT3 in the TSPG groups except 36h group had significant time-dependent relation(P<0.05),and there was significant deviation between the control group and the TSPG groups(P<0.01). Laser Confocal showed the green Fluorescent protain districted in the cytoplasm like bud, a little in the nucleus in the control group, but increased in the nucleus and decreased in the cytoplasm when induced by TSPG for 12h,and no significant deviation between the control group and 36 h group(P>0.05).Conclusion: The TSPG can induced the expression of STAT3 in the nucleus in K562 cells, and The expression and distriction of STAT3 in the TSPG groups had significant time-dependent relation; TSPG may plays an essential role in the promoting hematopoietic JAK-STAT Signals pathway. Objective:To study the artesunate water extract on the proliferation,cell cycle distribution, apoptosis of A549 and MCF-7 cell lines.Methods : Artemisinin residual was extracted in Water Solvent. Extraction was Purified and crystallized. A549 and MCF-7 cells were divided into 6 groups according to the concentration of drug. Proliferation assay was tested by MTT method on A549 and MCF-7 cells as described above and all treated for 24,48,72 hours. Cell cycle distribution, apoptosis of A549 and MCF-7 cells treated with drug were determined by using flow cytometry analysis after 48 hours.Results:Inhibition rations of A549 and MCF-7 cells treated with drug had significant time and concentration-dependent relation(P<0.01).FCM analysis showed drug concentration had positive correlation with A549 and MCF-7 cells in G0/G1 cell cycle phase and a inverse correlation with which in G2/S phase after treated with drug for 48hours; the rate of cell apoptosis raised in drug group, especially in high drug concentration(100μg/ml).Conclusion:Water extract from Artemisinin residual can effectively inhibit A549 and MCF-7 cells proliferation and accumulate in G0/G1 cell cycle phases.