Study On The Differential Proteomics Expression Of Glioblastoma Cell Lines Derived From Chinese

Background: Glioma is the most commonest intracranial malignant tumor. Glioma cells proliferate rapidly.lt has no obvious clinical symptoms in early stage. So many patients seek medical treatment till late stage.Because of its character of invasive growth, no obvious boundaries between normal brain tissues, the majority is not limited to one brain lobe, surgery often can not totally resect tumor tissues, even if combining radiotherapy and chemotherapy, its clinical effect is still poor, the recurrence rate and mortality is still high,it seriously endanger human health. Early detection and identification of glioma has great significance for glioma therapy. With the development of imaging, clinical diagnosis of glioma has made considerable progress, but the biological diagnosis of glioma is still lack of effective means, resulting in this situation is mainly due to lack of sensitive, specific biological markers. Proteomics has developed rapidly in recent years, and it will provide a new technology platform for finding many new tumor biological markers. And early diagnosis and identification depend on specific tumor biomarker discoveries of glioma specimens, plasma, cerebrospinal fluid. Human serum protein expression profiling study is rare at home and abroad, may be due to the blood-brain barrier leading to specific tumor protein expression in serum is very slow.Glioma cell metabolites secrete directly into the cerebrospinal fluid, so the specific protein in cerebrospinal fluid reflect glioma metabolism changes dynamically, the protein component analysis of cerebrospinal fluid may find a specific tumor markers. However glioma biomarker research is still most wide using tumor specimens or cell lines for analysis to find tumor markers for diagnosis, distinguishing benign or malignant tumor.We conducted proteomics research on malignant glioma cell lines in order to find much more specific proteins which participate in the malignant evolution of glioma, it will be helpful to further elucidating the pathogenesis, looking for therapeutic targets, screening clinical drug and developing new drugs.Objective:To establish the differential proteomics expression profiles of malignant glioma cell lines from Chinese, and provide reference for other basic related researches. To find key proteins as much as possible which participate in the malignant evolution of glioma from Chinese, and provide theoretical basis and reference data for elucidating the pathogenesis of gliomas, looking for therapeutic targets, screening clinical drugs and developing new drugs.Methods: The total protein was extracted from 3 gliobma cell lines, named CHG-5, TJ899 and TJ905. After an even mixing, the total protein was presented by two-dimensional (2D) electrophoresis, scanned and analyzed. Part of the identified protein spots were verified by immunocytochemistry. The glia cells were used as control through the study.Results:13 differential protein spots were selected,5 protein expression level decreased, eight protein expression level increased,and 10 protein spots were identified successfully, which probably involved in cytoskeleton forming, cellular metabolism, tumor migration, stress and inflammatory reaction. These results were coincident with that from immunocytochemistry.Conclusion:There were obviously differential proteomics expression between glioma cell lines and normal glia cells, and they may play key roles in the transform progress of glia to glioma.