Coblation Nucleoplasty In Cervical Intervertebral Disc Its Application Foundation Experimental Research

Objective: The research to sheep cervical intervertebral disc CN, discusses CN the potency and the security, provides the theory basis for the clinical security and the effective application.Methods: Use 7 fresh 18-month-old sheep slaughtered of freshly cervical spine, takes 40 sheep intervertebral discs, stochastically divide into the experimental 3 groups (each group of 10 cervical intervertebral disc) and the control group 1 group (10 disc), the experimental group were used 80W (1 grade, A group), 100W (2 grade, B group), 120W (3 grade, C), cures have CN, time 20s, furthest point away from the disc is located in the middle of posterior edge of 5mm,simulated operation of the control group, no Ablation. Intraoperative measurement the anterior border of intervertebral disc,hinder margin of intervertebral disc and foramen intervertebrale place temperature,measured after CN disc in the form of cross-sectional area ablation cavity size,the statistical analysis. And to takes CN of the corresponding intervertebral stage two-sided ganglia line use immunohistochemieal S-P to investigate Heat shock protein70(HSP70) expression.Results: Each of the experimental group in all, experiment sheep cervical intervertebral disc nucleus pulposus ablation vaporization cavity cross-sectional diameter and area compared with the control group were statistically significant (p<0.05); A group compared with the control group the difference no statistically significant (p>0.05). CN can form the effectively ablation vaporize cavity in the experiment sheep cervical intervertebral disc nucleus pulposus.Each of the experimental group in all, the leading edge of vertebral body,posterior edge of vertebral body and intervertebral foramina temperature compared with the control group were statistically significant (p<0.05).CN temperature change and ablation vaporization cavity cross-sectional area and CN energy output was positively related(p<0.05).C group emergence injury necrosis changes of pathological and HSP70 positive expression, the positive rate of 95% (19/20), the control group and the A, B group of HSP70 negative expression.Conclusion: 1.CN can form the effectively ablation vaporize cavity in the experiment sheep cervical intervertebral disc nucleus pulposus, thereby reducing the intradiscal disc internal pressure, can effective treatment the cervical intervertebral disc herniation.2.CN in the experimental sheep cervical intervertebral disc,time 20s,below energy output 100W, don't lead to the sheep cervical spinal ganglion cells heat damage; CN energy output 120W, lead to the sheep cervical spinal ganglion cells heat damage .3.Cervical spinal percutaneous anterior road CN when chooses 2 grades (energy 100W),operative time 20s,the ablation vaporization reduced pressure cavity forms accurately. cutter is located at the farthest point from the middle of intervertebral disc posterior edge 5mm place, the nervous tissue is safe.