| Objective: To investigate the effect of RhoA/Rho-kinase pathway on the expression of Klotho (KL) protein in the brain choroid plexus in mice.Methods: 24 male kunming mice were divided randomly into three groups: the normal control group, NOS blockade (L-NAME) group, and L-NAME plus fasudil group,and were administered by corresponding medicine respectively, for successive four weeks. The expression of KL protein in the brain choroid plexus were measured by Immunohistochemistry and Western blotting. The change of expression of RhoA/Rho-kinase mRNA were detected by RT-PCR.Results: Compared with the control group, the expression of RhoA/Rho-kinase mRNA increased significantly (P<0.01) and the expression of KL protein decreased sharply (P<0.01) in the L-NAME group. Fasudil can reverse this changes(P<0.01). Conclusions: These results suggest that L-NAME could induced downregulation of expression KL protein via activated RhoA/Rho-kinase pathway in mice brain. Objective: To investigate the effect and the mechanisms of Telmisartan on the expression of Klotho protein in the brain choroid plexus in mice.Methods: 32 male kunming mice were divided randomly into four groups: the normal control group, NOS blockade (L-NAME) group, Telmisartan group and L-NAME plus Telmisartan group,and were administered by corresponding medicine respectively, for successive four weeks. The expression of KL protein in the brain choroid plexus were measured by Immunohistochemistry and Western blotting. The change of expression of RhoA/ ROCK mRNA were detected by RT-PCR.Results: Compared with the control group, the expression of ROCK mRNA increased significantly (P <0.01) and the expression of KL protein decreased sharply (P<0.01) in the L-NAME group. Telmisartan can reverse this changes(P <0.01).Conclusions: These results suggest that Telmisartan may interfere with L-NAME-induced reduction of KL protein expression, the mechanism may be related to inhibit RhoA/ ROCK signaling pathway activity. |
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