| Objective:To explore effect of Buyang Huanwu Decoction (BHD)and its active fractions such as alkaloid and glycoside extracted from BHD on arterial thrombosis in rats and rat platelet aggregation and cAMP of the platelet and cGMP of the platelet. Research antithrombotic form mechanism of Buyang Huanwu Decoction (BHD)and its active fractions from the angle of platelet activation and reveal substantial foundation on antithrombotic form and mechanism of antiplatelet activation about BHD.Methods:1.Inbody experiment:Carotid thrombosis model in rats was induced by topical ferric chloride. The model rats were administered with BHD,alkaloid,glycoside and ticlopidine respectively. Weight of thrombosis was determined in each group.The levels of TXB2 and 6-keto-PGF1αin plasma in rats were measured by radioimmunoassay. 2.Outbody experiment:Divide SD rats into five groups,inclouding BHD group,alkaloid group,glycoside group and ticlopidine group.Drug doses,time and means of administering drugs are the same to experiment 1.Blood result from nearside carotid, PRP is abstracted by acentric means, number of platelets are acculated and experiment of platelet aggregation induced by ADP is carried out. The levels of cAMP and cGMP resulting from blood plasma in rats before and after platelet aggregation were measured by radioimmunoassay.Results:1.After model group made model, carotid had significant thrombus form.Weights of thrombosis in BHD group, alkaloid group, glycoside group and ticlopidine group were significantly lower than that of model group(p<0.05).2.The levels of TXB2 were increased and the levels of 6-keto-PGF1αwere decreased markedly in model group(p<0.05). The levels of TXB2 in BHD group were markedly higher than that of the sham-operated group (p<0.05),and approachable to that of the model group(p>0.05). BHD group could increase the levels of 6-keto-PGF1αwhen compared to the model group(p<0.05). Compared with the model group, alkaloid group could decrease the levels of TXB2 and increase the levels of 6-keto-PGF1αsignificantly(p<0.05). The tendency of the decreased TXB2 was noted in glycoside group, but there was no significant difference in TXB2 between glycoside group and the model group(p>0.05).The levels of 6-keto-PGF1αin glycoside group were lower than that of the sham-operated group(p<0.05), and approachable to that of the model group(p>0.05).3.After platelet aggregation inhibitations of BHD,alkaloid and glycoside groups were markedly lower than that of blank group (p<0.05).4.Compared with cAMP before platelet aggregation,cAMP of blank group,BHD group, glycoside group and Ticlopidine group after platelet aggregation could be decreased significantly(p<0.05).The tendency of the decreased cAMP after platelet aggregation was noted in alkaloid group, but there was no significant difference before platelet aggregation (p>0.05).Compared with decreasion of cAMP in every group before and after platelet aggregation, there was no significant difference (p>0.05).5. cGMP of blank group and BHD group after platelet aggregation could be decreased significantly(p<0.05).The tendency of the decreased cGMP after platelet aggregation was noted in alkaloid group,glycoside group and Ticlopidine group, but there was no significant difference before platelet aggregation (p>0.05).Compared with decreasion of cGMP in every group before and after platelet aggregation, cGMP of alkaloid group after platelet aggregation could be decreased significantly than that of blank group (p<0.05). when compared to the blank group,there was no significant difference in glycoside group,BHD group and Ticlopidine group (p>0.05).Conclusions:BHD had effections of Antithrombotic form.Maybe alkaloid and glycoside are main substantial foundation on antithrombotic form. The efficacy of alkaloid may contribute to inhibiting production of TXA2 and increasing synthesis of PGI2. Antithrombotic action of glycoside was not based on its modulation on TXA2 and PGI2,it may be concerned with other aspects.In resarch of effection of platelet aggregation,every medicine could inhabit platelet aggregation by ADP. Effection of antiplatelet aggregation of alkaloid was relative to inhabition of cAMP and cGMP when platelet was aggregated. Antiplatelet aggregation action of glycoside was not based on increasement of cAMP and cGMP in platelets,it may be concerned with other aspects.Result reveals that alkaloid may be main substantial foundation on antiplatelet action and may be relative to inhibiting production of TXA2, producing synthesis of PGI2 and increasing cAMP and cGMP in platelets. |
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