Ornithogalum On Hepatocellular Carcinoma In The Development Of Vegf, Cyclind1 Expression And Effect

Objective:To explore the relationship of Ornithogalum Caudatum Ait saponins and the expression of VEGF,and CyclinDl in liver cancer. To explore a new way of Tradtional Chinese medicine prevent liver cancer.Methods:Ornithogalum Caudatum Ait saponin extract, diethylnitrosamine induced hepatocarcinogenesis. Ornithogalum Caudatum Ait saponins interfere with, the use of conventional HE staining and pathological changes in liver tissue. Observed by immunohistochemistry and analyzed the liver tissue of vascular endothelial growth factor (VEGF), cyclin Dl(CyclinDl) expression.Results:(1)12 weeks of model group:microscope features of diverse structural damage to the liver nodules and the formation of false lobules interstitial infiltration of inflammatory cells, parenchymal cells is more extensive nuclear abnormalities and degeneration hyaline degeneration of the water, cut into the more common L. cytoplasm, some nodules can be seen around the small focal changes.12 weeks to prevent groups:microscope features with no hyperplastic nodules, a small amount of fake leaflets, some small amount of fine fiber formed at intervals, spotty necrosis of liver cells, hyaline degeneration of water, the cytoplasm of L. by more common. 14 weeks prevention group:microscope with no nodules, lobular formation of irregular fiber spacing false, hepatocyte degeneration fatty degeneration of the water, a few inflammatory cells were more common.14-week treatment group, liver cirrhosis: endoscopic part of the more common cirrhosis, cell water variability, part of the bile duct epithelial hyperplasia.14 weeks of model group:total destruction of hepatic lobules, liver nodules increased cell proliferation, lobular collapse and disappearance of liver plate, with hemorrhage more common. Addicted to reduce the formation of liver cell proliferation and transparent lesions, nodules with marked hyperplasia of the liver cell atypia, uniform cytoplasmic staining, mitotic more common. Disordered and irregular arrangement of hepatic cells, showing cell clusters and clear cell hyperplasia; part of the liver nuclei enlarged, nucleolus increased vacuolization of the nucleus, nuclear phenomena are off.18 weeks liver cancer treatment group:a small amount of microscopic nodules appear fibrosis, a small amount of fake leaflets, local hepatic lobules recovery, more common in early cirrhosis. (2) Hardening stage of liver injury prevention group at 12 weeks the highest expression of VEGF LI, and the corresponding model group at 12 weeks between the two groups was not significant; induced cancer model group LI value of 14 weeks is relatively low, and 14 weeks,12 weeks to prevent between the two groups had significant differences; 14 weeks of the model group and model group at 12 weeks and 18 weeks of model group, treatment was significantly different between the two groups; 14 weeks of model group, model group and the corresponding 18-week treatment groups showed no statistical significance; 14 weeks to 12 weeks of preventive treatment group and model group,18 weeks of treatment between the two groups was statistically significant.14 weeks induced cancer model group CyclinDl LI expression in the highest 14 weeks of the model group and its corresponding prevention group, treatment groups were statistically significant, with 18 weeks of treatment group, a model was statistically significant between groups; 14 weeks of the model group and 12 weeks prevention group, model group, no statistical significance; 12 weeks prevention group, model group LI expression were higher, and 14 weeks prevention group and treatment group and 18 weeks of treatment group, the model was statistically significant between the two groups.Conclusion:In den-induced hepatic lesions stage (14 weeks), Ornithogalum Caudatum Ait can interfere with VEGF precancerous lesions in the liver expression; liver lesion stage (14 weeks) of VEGF is low, probably and VEGF receptor affinity strength related need for further research.2. At the den-induced liver injury before hardening stage, liver disease stage CyclinDl LI values decline. Ornithogalum saponins in liver injury before hardening stage, liver disease stage can reduce the expression CyclinDl, plays the role of the tumor. In den-induced liver cancer of liver injury hardening phase, the expression CyclinDl is higher, CyclinDl may be early molecular events.