| Objective:Esophageal carcinoma is a kind of common maglignant tumor in china.It was the fourth in the death rate of malignant tumor in our country.Themost.The research has shown tha the carcerrization of epidermis of mueous membrane of People's esophagus is along-temr, multistage and progressing process of development. the cancerization mechanism of esophageal carcinoma is closely related to the proliferation, differentiation and disorder of decease and regulation & control of cells.Many kinds of cancer genes and tumor cheeking genes take part in the occurrence & development of it. The hysiology function of proto oncogene causing various,incitement and more c-fos and c-jun are the cancer genes which code to check nuclear protein.belong to the early gene this kind of gens is subjected to immediately express by various incitement and produce to transcript factor.This kind of gene adjust to contro ltranseription and expresstion of other geneses and regulate the growth, development and division of normal cell. And have shown that the expressions of c-fos and c-jun are related to the classification of tumrors, clinical stages and the soakage & metasastis.The research of both home and abroad has also shown thatpoliferating cell nuclear natigen(PCNA) is an effectiven symbol of the malingantTumor cells in the period of proliferation.The studies of livesr, stomach, galactophore and intestinum crassmu have shown that the expressions of PCNA are related to the classification of tumrors, clinical stages and the soakage & metasastis.To investigate the expression of c-fos,c-jun and PCNA inprimar esophageal squamous cellcarcinoma and to evaluate whether these Alterations could provide clinical significant informations. Methods:Select wax lumps were cutted off by 2004 at the xinjiang medieinedepartment University the second affiliated Hospital,The expression of c-fos, c-jun and PCNA was studied by StrePtavidin Peroxidase(SP) immunohisto chemical methodwas used to detect the expressions of c-fos,c-jun and PCNA and to analyse the correlation between them and different clinical pathological features in 60 cases of esophageal squamous cell carcinoma, in 20 cases of abnormal and in 10 cases of normal esophageal tissues.Results:(1) The positive frequency of c-fos in 60 cases of esophageal squamous cell carcinoma, in 20 cases of abnormal and in 10 cases of normal esophageal tissues was respectively 56%(51/60),75%(15/20)and 0, The group had significant difference (P<0.05). The positive frequency of c-jun was respectively 38%(23/60),55 %(11/20)和10%(1/10),The group had significant difference (P<0.05).But the positive frequency of PCNA was respectively 70%(42/60),50%(10/20) and 10%(1/10) The group had significant difference (P<0.05).(2) The positive ratio of c-fos, c-jun and PCNA proteinwas significantly higher in thewell differentiated and moderately differentiated esophageal carcinomas than in the poorly differentiated esophageal carcinomas(P<0.05). The esophageal carcinomas with lymph node metastasis expressed c-fos, c-jun and PCNA protein more markedly than those without lymphnode metastasis(P<0.05). (3) In carcinoma samples, the expression of c-fos protein was Positively correlated with that of c-jun(spearman rho 0.399, P<0.05). (4) the expression of c-fos,c-jun and PCNA at the preoperative biopsy and afteroperative pathology tissue were no significant relationship with either clinical stage (P>0.05).Conclusions:The overexpression of c-fos, c-jun and PCNA could serve as an important index in the aspect of invasiveness and metastasis in esophageal squamous cell carcinoma,and might be used as valuable molecular markers for the tumor progression and diagnose. |
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