Study On The Value Of Combinded Immunoprotection With Schistosoma Japonicum Ribosomal Protein S4 Gene And Recombinant Protein

Objective:To study the protective efficacy of co-immunization with Schistosoma japonicum ribosomal protein S4(SjRPS4) gene and recombinant protein vaccine against Schistosoma japonicum in mice.Methods:All mice were immunized three times with plasimids pcDNA3. 0/SjRPS4, protein of SjRPS4 or both the DNA and protein boosting vaccination in an interval of 2 weeks, and challenged each with 20 cercarie of Schistosoma japonicum at 2 weeks after final immunization. At the 45th day after challenge, all mice were sacrificed, and the numbers of worms and eggs in the liver, Intestine, feces and uterus tissue were counted.Results:Compared with the normal saline group, the worm reductions, eggs reductions in the feces, uterus, liver and Intestine tissue of the DNA priming-protein boosting vaccination group were 49.7%,62.6%,46.0%,49.0% and 54.3%, they were significantly higher than that in SjRPS4 DNA group (the worm reductions, eggs reductions in the feces, uterus, liver and Intestine tissue were 36.2%,48.7%,35.2%, 41.8%,40.5%) (P<0.05) and in recombinant protein group (the worm reductions, eggs reductions in the feces, uterus and Intestine tissue were 38.2%,50.1%,38.9%, 42.4%) (P<0.05).Conclusion:SjRPS4 DNA and recombinant protein vaccination could induce partial protective immunity against Schistosoma japonicum in mice. The DNA priming-protein boosting vaccination regimen was better than the single vaccination.