Glutathione S-transferase Enzyme Gene Polymorphism And Glutathione To The Mechanism Of Oxidative Stress Parameters And Coronary Heart Disease

Part 1: Association of GSTM1 and GSTT1 gene polymorphisms with coronary artery disease in relation to tobacco smokingThe GSTs represent an important superfamily of Phaseâ…¡xenobiotic-metabolizing enzymes that catalyze the conjugation of reduced glutathione to these potentially hazardous reactive electrophilic species to detoxify them and facilitate their excretion. Recent studies suggest that the common variant in the GSTM1 and GSTT1 gene is associated with the risk of smoking-related coronary artery disease. Intra-ethnic as well as inter-ethnic differences are known to impact the frequencies of GST gene polymorphisms, thus influence its interactive effect with tobacco smoking on CAD risk. The present study was conducted a case-control study to evaluate the interaction of the genetic polymorphisms of GSTM1 and GSTT1 with cigarette smoking in the risk of CAD in Chinese population.1. The distribution of genotypic characteristics in the cases and controls and the risk estimates for the variant GSTM1/GSTT1 genotypesThe homozygous deletion of GSTM1 had a frequency of 32.1% among patients with CAD and 21.3% among those without CAD (P = 0.004). The frequency of the GSTT1 null genotype was 27.8% among the patients with CAD and 19.1% among CAD-free subjects (P = 0.016).2. Association of GSTM1/GSTT1 polymorphism with the severity of CAD among casesPatients who smoke having both of the wild genotypes of GSTM1 and GSTT1 were protected in developing coronary heart disease.( P<0.001) Moreover, smokers with combined GSTM1nullGSTT1null genotypes had significantly higher number of stenosed vessels than those with the positive genotypes (p = 0.02). Part 2: The common variant in the GSTM1 and GSTT1 genes is related to markers of oxidative stress and inflammation in patients with coronary artery disease: a case-only studyRecent studies suggest that the common variant in the GSTM1 and GSTT1 genes modifies the risk of coronary artery disease. An interaction between GSTM1/GSTT1 genotypes and CAD needs further investigation. Our study used PCR protocols to evaluate the prevalence of GSTM1/GSTT1 gene polymorphisms, and levels of CRP, FIB, TAOS, GSH and WBC were measured, to provide insight into the role of GSTs genetic variant and markers of oxidative stress and inflammation in CAD patients.1. Levels of oxidative stress and inflammation markers in different GSTM1/GSTT1 groupsWe found subjects with combined GSTM1nullGSTT1null genotypes had higher CRP and FIB and lower TAOS compared to patients with wild-type GSTM1/GSTT1 genes.2. Association of markers of oxidative stress and inflammation with the severity of CADA stepwise decline in levels of TAOS and GSH, as well as the prevalence of wild-type GSTM1 and GSTT1 was found depending on the number of affected vessels, while there was a trend of higher FIB and WBC.