Tgf-¦Â1 In The Different Levels Of Cervical Exfoliated Cells And Its Significance

Objectivesl.The aim of this study is to investigate the expression level of Transforming growth factor-β1(TGF-(31) in cast-off cells of cervical intraepithelial neoplasia (CIN).2.Meanwhile compare the expression level of HPV(+) with HPV(-),to see TGF-β1 in carcinogenesist whether the occurrence and development of a synergistic effect with HPV infection.3.To evaluate the expression level of TGF-β1 in cast-off cells of cervical intraepithelial neoplasia,it wether correspond with the expression level on cervical tissues.Subjects and MethodsCollect 89 TCT specimens which result above ASC-US in Department of Gynecology in First Hospital of Shanxi Medical University, during October 2009 to December 2010, meanwhile that have result of HPV-DNA and cervical biopsy. ASC-US-HPV(+)18 ASC-US-HPV(-) 17,LSIL-HPV(+) 20,LSIL-HPV(-) 9,HSIL-HPV(+)18,HSIL-HPV(-) 7.Collect 89 paraffin distillate specimens which correspond abnormal TCT, CINⅠ-HPV (+)21,CINⅠ-HPV(-) 27,CINⅡ-HPV(+) 16,CINⅡ-HPV(-) 4,CINⅢ-HPV (+) 19,CINⅢ-HPV(-) 2.10 normal cervical biopsy specimens including the TCT is used as controls.Using immunohistochemical to investigate the expression level of Transforming growth factor-β1(TGF-β1) in cast-off cells of cervical and cervical biopsies.Using SPSS 17.0 for statistical analysis.Results1. Expression of TGF-β1 decreased as tumor cells progressed from cervical intraepithelial neoplasia, (P＜0.05)2. Furthermore, we observed that cervical lesions with HPV infection expressed significantly less TGF-β1, (P＜0.05)3. There was a high incidence of cervical pre-malignant lesions(CINⅡ, CINⅢ) in the cases with negative expression TGF-β1 and in the cases with HPV(+)-TGF-β1(-), as the gold standard on the basis of pathological diagnosis in the specimens, while in another condition the incidence was low, (P<0.05)4. Expression of TGF-β1 in paraffin distillate of cast-off cells of cervical may be greatly reflect the situation of the biopsy tissue of cervical,(P>0.05).Conclusions1. Our result showed TGF-β1 acts as an antiproliferative factor in normal epithelial cells and at early stages of oncogenesis. 2. Meanwhile progressive loss of epithelial TGF-β1 expression and synthesis may be important in HPV associated human cervical carcinogenesis.3. There was a high incidence of cervical pre-malignant lesions(CINⅡ, CINⅢ) in the cases with negative expression TGF-β1 as the gold standard on the basis of pathological diagnosis in the specimens.4. Expression of TGF-β1 in paraffin distillate of cast-off cells of cervical may be greatly reflect the situation of the biopsy tissue of cervical.