| Dipsacus asperoides (also known as"Guchuicao", "Heshangtou") was first recorded in the "Shen Nong Ben Cao Jing "and is listed as the top grade.it's clinical actions are curing the pain in waist and leg Bruises, beriberoid disease,Metrorrhagia. Dipsacus asperoides is the traditional medicine and have large amount in clinical and good efficacy.,it was commonly used in orthopedic and gynecological. With the deepening study, Dipsacus asperoides was found have antioxidant, anti-aging and improve the pharmacological activity of learning and memory, with great research value.The Chinese Pharmacopoeia(2010 version) provide the content of AsperosaponinⅥis not less than 2.0%Ⅵ,to evaluate the quality of Dipsacus asperoides only with one chemical consitituent is One-sided,and the paper studied the chemical constituents of effective fraction of Dipsacus asper by extractiing, separating, purifing in order to provide the Standards for the quality study,and fourther more to provide scientific evidence for chemical consitituent study, quality standard control and clinical application of Dipsacus asperoides.The study is mainly divided into the following parts:Part one:Reviewed the research work of Dipsacus asperoides about chemical consitituents, pharmacological actions and clinical practices,49 references were cited in all.Part two:To study the chemical constituents of effective fraction of Dipsacus asper by extractiing, separating, purifing. First,With 70% ethanol extract, and then enriched and segmentation with D101 macroporous resin,and then, silica gel, RP-C18 silica gel, Sephadex-LH20 gel, HPLC preparation methods were applied, TLC method and HPLC method were adopted to trace detection at the same time.6 compounds were separated According to physical and chemical properties of compounds, and ultraviolet, infrared, mass spectrometry, NMR and other spectral identification,the 6 compounds were Loganin, Daucosterol, Sweroside, Asperosaponin VI,Dipsacoside B, another compound has been identified as triterpenoid saponins, the structure is spectrumunfolding.Part three:The study of Quality standard for Dipsacus asperoides1 Vanillin-perchloric acid color method was adopted to determine the total saponins of Dipsacus asperoides.First, The action condition was optimized by orthogonal analysis method.Then,Determination of total saponins was determined by the Ultraviolet-visible spectrophotometry at 550nm.52 samples from south area of china were determined in all.2 Loganin,Sweroside,AsperosaponinⅥwere used to be the Chemical Markers to establish the multi-component, multi-index determination method of Dipsacus asperoides by HPLC method.52 samples from south area of china were determined in all. The cluster analysis of the samples was done by DPS software, the samples wers divided into four categories.3 To establish a new HPLC chromatographic fingerprints method of the Dipsacus asperoides and compare the fingerprints of Dipsacus asperoides collected from different regions so as to set up a specific method for controlling the quality of Dipsacus asperoides. Ninteen batches samples of different regions were analyzed with the HPLC-UV. Ultimate XB-C18 (250 mm×4.6 mm,5μm) Column was adopted, the mobile phase was comprised of Acetonitrile(A) and water with 0.05%H3PO4 buffer solution (B) in gradient elution mode. The column temperature is 25℃, with the flow rate of 1 mL-min-1 and the detective wavelenth is 212 nm. The mutual mode of fingerprints was set up with "Similarity evaluation system for chromatographic fingerprint of TCM" (Version 2004 A) and 21 characteristic marks were marked. The similarity of different samples was compared.4 Completed the drafting and the notes of drafting of the quality standard of Dipsacus asperoides... |
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