| ObjectiveThe research aims at observing the effect on Ach, NOS, cGMP on Ach-NO-cGMP singal transduction pathway of stress-induced hypertension rats treated by twirling reinforcing and reducing manipulation, revealing the mechanism of relativity between twirling reinforcing and reducing manipulation and the different biology effect, providing an experimental evidence to twirling reinforcing and reducing manipulation, and promoting the popularization of manipulation in acupuncture practice.Method60 clean male Wistar rats with weight ranging from 220g to 280g were divided into A(blank group), B(model group), C(needling group), D(reinforcing group), and E(reducing group) five groups randomly. Electric shock and noise were applied to stimulate the rats into stress-induced hypertension models except group A. Each rat was treated by 1 minute manipulation and 20 minutes needle retaining. The treatment lasted for 7 days and once each day. After the treatment Ach, NOS, cGMP contents in plasma and aortic were detected by means of radio immuno assay and ELISA.Result1 Systolic blood pressure changes of stress-induced hypertension rats after electric shock and noise stimulation and acupuncture treatmentThe systolic blood pressure of each stimulated group increased significantly after 15 days lectric shock and noise stimulation. Campared with group A the difference were significant (p<0.01). The differences of each stimulated group were not obvious (p>0.05). After 7 treatment days, the systolic blood pressure of group B were stable and different to the group A (p<0.01); the the systolic blood pressure of group C, group D and group E all decreased, the group E were the most obvious, and the group D and group C were sinificient different to group E (p<0.05); group D and group E campared with group B were obviously different (p<0.01); and the differences of systolic blood pressure were significant between group C and group B (p<0.05).2 Changes of Ach in plasma of stress-induced hypertension ratsThe Ach in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the Ach of group C,D and E all increased and different to group B (p<0.01); the Ach of group E inceased most obviously, which were different to group C and group D (p<0.01); however, there were no signifcient difference in group C and D (p>0.05). 3 Changes of NOS in aortic of stress-induced hypertension ratsThe NOS in aortic decreased significantly in group B, which were obviously different to group A (p<0.01); the NOS of group C,D and E all increased, there were significient differences between group C and group B (p<0.05); campared group D and E with group B, the differences were obvious (p<0.05), in which the NOS in group D was the highest; there were different between group D and group C (p<0.05); however, there were no signifient differences between group E and C and group D and E (p>0.05).3 Changes of cGMP in aortic of stress-induced hypertension ratsThe cGMP in plasma decreased significantly in group B, which were obviously different to group A (p<0.01); the cGMP of group C,D and E all increased, in which the group D was the highest; campared group D and E with group B, the differences were obvious (p<0.01) campared group C and group E the differences were signifcient (p<0.05); campared group C and group D the differences were more signifcient (p<0.01); however, there were no signifient differences between group E and C and group D and E (p>0.05)Conclusion1 Twirling reinforcing and reducing manipulation could reduce stress-induced hypertension, the antihypertensive effect are difference between twirling reinforcing and reducing manipulation;2 Twirling reinforcing and reducing manipulation could increase the contents of Ach, NOS, cGMP in plasma and aortic of stress-induced hypertension rats;3 Ach-NO-cGMP singal transduction pathway could one of the blood pressure regulating mechanism of twirling reinforcing and reducing manipulation. |
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