Allogeneic Hematopoietic Stem Cell Transplantation Human Herpes Virus Infection

Establish a Real-time fluorescent quantitative PCR method for quantifying the human herpesvirus 7(HHV-7), measure clinical specimens, and study the infection of allogeneic hematopoietic stem cell transplantation (allo-HSCT).1 Establish quantitative human herpesvirus 7 with Real-time Fluorescent Quantitative PCRObjective To establish a Real-time fluorescent quantitative PCR method for quantifying the human herpesvirus 7(HHV-7). Methods According to the HHV-7 gene sequence, we designed and synthesized PCR primer and probe. The purified PCR product was sequenced after connecting with pGM-T plasmid. Standard recombinant plasmid extracted from the positive bacterium clone was used as standard substance. The sensitivity and specificity of the real-time fluorescent quantitative PCR were analyzed. Results Agarose gel electrophoresis and the sequence result indicated that the cloning successed. And The correlation coefficient of the standard curve was 0.998869. The amplification efficiency of PCR was 95.8565%,detection sensitivity was 10 copies/μL..Conclusion The method of quantification of HHV-7 with real-time fluorescent quantitative PCR is successfully established, and the method has good sensitivity and specificity, which can be used to quantitative HHV-7.2 Study the infection of allogeneic hematopoietic stem cell transplantationObjective To study the prevalence of human herpesvirus 7(HHV-7) infection inrecipients following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods 384 peripheral blood samples were colected before and weekly after allo-HSCT from 40 consecutive recipients,40 peripheral blood samples were were collected from 40 donors. Real-time Fluorescent Quantitative PCR was used to monitor HHV-7 DNA. Result DNA positive rate of 40 allo-HSCT donors'specimens was 32.5%(13/40),The median of virus load was 271EqCop/106PBC (37～1095);DNA positive rate of 40 allo-HSCT recipients' specimens colected before transplantation was 37.5%(15/40), The median of virus load was 285EqCop/106PBC (47～3764);344 specimens of 40 allo-HSCT recipients were colected after transplantation, DNA positive Rate was 43.90%(151/344), The median of virus load was 457EqCop/106PBC (41～5218), virus load of allo-HSCT recipients' specimens colected after transplantation was significantly higher than allo-HSCT donors (p<0.05). No relationship was observed between HHV-7 infection and gender, age, diseases. There was relationship between HHV-7 infection of transplanted recipients and Donors, untransplanted recipients(p<0.05). Conclusion HHV-7 infection of allo-HSCT patients is common。If virus load of Donors, untransplanted recipients is high, recipients were more prone to infect after transplantation.