Dendrobium Nobile Chemical Composition And The Hypoglycemic Effect Of A Preliminary Study

Dendrobium nobile Lindl. has been used as one of the materials of Shihu, a valuable traditional Chinese medicine for a long time. It is an epiphytic herb in Dendrobium Orchidaceae. The main effects of Shihu in traditional Chinese medicine theory are nourishing yin and clearing heat, tonifying stomach and promoting fluid. Now the research is is focused on rapid propagation and chemical. But the research on product is not so much. So this paper is about to study on product (medicine or health food).In this paper, Dendrobium nobile Lindl. which was plant in the standard plant base of Guizhou was used as the material.Part one:literature and summarizationsDocuments summarize the present research situation in chemistry, pharmacology of Dendrobium nobile Lindl.69 reference are cited.Part two:effect of water extracted from Dendrobium nobile Lindl. on STZ-induced hyperglycemia in mice.All of kinds of dose(WE(0.25g/kg), WE(0.5g/kg), WE(0.75g/kg)) water extracted from Dendrobium nobile Lindl. on STZ-induced hyperglycemia In mice have significant lowering blood-glucose effect.All of kinds of dose(WE(0.25g/kg), WE(0.5g/kg),WE(0.75g/kg)) water extracted from Dendrobium nobile Lindl. on STZ-induced hyperglycemia in mice do not have effects on body-weight of miceAll of kinds of dose (WE(0.25g/kg), WE(0.5g/kg), WE(0.75g/kg)) water extracted from Dendrobium nobile Lindl. in mice do not have effects on body-weight and blood-glucosePart three:the chemical constituents of Dendrobium nobile Lindl.In our study on chemical constituents of Dendrobium nobile Lindl.,7 compounds were obtained from the 70%ethanol extract of stems of through various chromatographic such as silica gel and sephadex LH-20. Their structures were elucidated on the basis of physico-chemical property and spectroscopic analysis.4 compounds were identified,which wereγ-quinide(JC002),1,7-dimethoxyxanthone(JC004), dendrobine(JC007), apige nin 6-C-α-rhamnoside-8-C-β-glucopyranoside(JC009), respectively. Compounds (JC002), Compounds (JC004), Compound (JC009), were isolated for the first from the Dendrobium genus.Part four:Content determination of dendrobine in Dendrobium nobil Lindl from different collection periods by Gas ChromatographyTo determine the content of dendrobine in Dendrobium nobil Lindl from different coLLection periods, to discover the inherent rule about the content change owing to different collection periods. The content of dendrobine varies in different harvest time, with 2.39%o at the lowest point in September and 5.59‰at the highest point in January. The dendrobine content of the annuals is 5.46‰, which is the largest among that of different harvest years. This offered evidence to determine the most appropriate harvest time for Dendrobium nobil Lindl...