| Anxiety is an emotional state, the patient's inner experience is the basic fear, such as fear, trembling, and even extreme panic or fear; this emotion is unpleasant and painful, can have an imminent collapse or faint feeling soon. Divided into panic disorder anxiety disorder and generalized anxiety (GAD) both. Typical symptoms of GAD include heightened vigilance, worry, irritability, increased muscle tension, tremor and heart palpitations and other independent activities change. Modern research has found that stress and anxiety are closely related phenomena, most patients with anxiety disorders had experienced severe stress events, so events can be anxiety Stress causes disease and incentives. Newer stress is defined as:the body of the uncoordinated, or the environmental stability is threatened. In recent years, researchers stress the in-depth exploration of effective treatment for stress, anti-anxiety drugs, primarily for the western benzodiazepine class, experiments show that the pivotal role of anti-anxiety drugs and excitement like GABA brain system and reduce the 5-HT system related activity. In recent years, found that there is brain specific benzodiazepine receptors drugs and a large number located in the hippocampus and other parts of western anti-anxiety drugs on the nerves but a lot of side effects. Anti-stress medicine in the field has highlighted the great potential. Ancient Chinese medicine, although not in the "stress" the term, but the ancient physicians had long recognized the environmental stimuli of various adverse effects on the body. Ancient physicians believe that environmental factors and strong or lasting psychological factors can cause the body to stimulate the body air-disorders, blood and viscera imbalance of yin and yang, balance and stable internal environment, causing diseases. The modern theory of psychological stress to the core of neuroendocrine-immune network, that in between the neuroendocrine and the immune system, there are a variety of neurotransmitters, neuropeptides, hormones and immune factors mediated interaction and regulation, in the overall level constitutes the neuroendocr ine network, neuroendocrine-immune network is the internal environment to maintain balance and stability and physiological function of the fundamental basis. Chinese medicine, "Liver Shuxie" function in the body stress plays an important role in regulating traditional Chinese medicine the liver is the body's core stress response. Therefore, the combination of modern medical research related to stress theory to explore further in the liver controlling dispersion and stress the leading role of traditional Chinese medicine in the prevention of chronic stress arising from the mechanism, not only conducive to the basic theory and clinical medicine research and development, but also for the prevention and treatment of many diseases of great importance. Xiaoyaosan to liver qi stagnation, spleen, nourishing the principle of the group side, since the self-created, its ancient physicians a lot of exposition and application, is still widely used in clinical subjects.Hippocampus is an important part of the limbic system, and the generation of emotion and motivation, and the higher nervous activity and the brain, such as learning and memory, respectively, and the parts of nerve function disorders in the pathogenesis of severe mood disorders may have an important role. Can inhibit the HPA axis stress response, and stress can contribute to hyperactivity of the HPA axis status returned to baseline levels. However, chronic stress can cause prolonged damage to the hippocampus, causing structural and functional changes, leading to the occurrence of affective disorders. Monoamine neurotransmitters, including:5-hydroxytryptamine (5-HT), norepinephrin e (NE) and dopamine (DA), they are both in the spirit of the classDisease pathogenesis, or the role of drugs in the spirit of the mechanism, have played an important role. Research Objectives:Therefore, this issue mainly in the hippocampus as the research object to chronic stress-induced anxiety-like behavior, and through high performance liquid chromatography of monoamine neurotransmitters in chronic liver Syndrome Model of restraint stress the hippocampus changes, combined with the dynamic changes of behavior, suggesting that chronic stress, and reveal the state of Xiaoyaosan regulation mechanism. Physiological function of liver and liver of TCM theory to provide experimental basis for controlling dispersion.Methods:1 adult male with a SPF level 25 SD rats (160±20) g.2 days feeding adaptation, according to open field test scores were divided into normal control group (A group),7 day group (B group),7 days Xiaoyaosan group (C group),21 day group (D group),21 days Xiaoyaosan group (E group),5 rats in each group. Approach to chronic restraint stress in rats made, the rats were specially bound in the shackles of the shelves,3 hours per day for 21 days. Since the beginning of mo deling 1, C, E groups before daily restraint 1h fed Xiaoyaosan, intragastric volume 1mL/100g weight. A, B, D group were fed with an equal volume of distilled water.2 activities in rats with a video monitor to observe the rat's mental state, binding reaction, stool texture.3 high performance liquid chromatography monoamine neurotransmitter expression.In the experiment, the author of the hippocampus as the main object of study.Because the main anti-anxiety drugs in the Western benzodiazepine class, there exist experimental results show that specific brain benzodiazepine receptor drugs, and a large number located in the hippocampus and other parts. And the left hippocampus in this experiment used only as research subjects, should be accessible in the literature the author found that the researchers used the process of local consistency (ReHo) as a measurement of hippocampal function, the results suggest that stress in the hippocampus than the normal value of the left ReHo control group, the difference was statistically significant. The right hippocampus with the control group did not change significantly. Tip of the stress caused by the dysfunction of hippocampus may exist side of the phenomenon. The researchers have also pointed out that the left hippocampus in patients with anxiety disorders were significantly higher than the normal amino acid neurotransmitters, and the right hippocampus in normal human neurotransmitter did not differ between patients with anxiety generated; and found that the left hippocampus in patients with anxiety and parahippocampal area significantly increased glucose metabolism, but no significant change in the right side. Confirmed in the pathogenesis of anxiety in patients about the existence of functional activity of hippocampal asymmetry. These two results suggest that anxiety disorders are left hippocampus may be involved in the pathogenesis of the disease, anxiety, there may be left hippocampus in patients with neuronal activity, metabolism of nerve cells and neurotransmitters conduction abnormalities. Therefore, experimental research subjects in this experiment, select the hippocampus-the left hippocampus of the study.Results:21 days treatment group compared with the control group,21 days of drug HIAA levels in hippocampus were significantly height difference (P<0.01).21 days, the model group compared with the control group, HIAA levels in the hippocampus were significantly higher (P <0.01),5-HT content also significantly different (P<0.05).7 days drug group compared with the control group at 5-HT levels increased significantly higher, with statistical difference (P<0.01); in the NE content of 7 days on the drug group increased compared with normal group, statistically significant differences (P<0.01).7-day group compared with the control group,5-HT in the hippocampus and both are on the NE content increased, there were significant differences (P<0.01), with statistical significance. In addition, with 21 days of model group and drug group compared to 21 days, two groups on the content of 5-HT there were significant differences (P<0.01), with statistical significance. Second, the 21-day group and 7 days of drug treatment group and 7 day group comparison, in the HIAA,5-HT and NE levels were significantly different on both (P<0.01), with statistical significance.Conclusion:Liver Syndrome Model hippocampus 5-HT, NE significantly increased. After treatment with Xiaoyaosan 5-HT, NE values were decreased,5-HIAA levels increased, suggesting that prevention and treatment of anxiety Xiaoyaosan mechanism may increase 5-HT and NE function and its conversion rate related. From the experimental results of the study and can be seen 7 days group and 21 group differences do exist, in the absence of drug intervention group compared with 21 days, 7 days a model model group,5-HT and NE content were decreased, which Chronic stress disorder may be one of the reasons may be the result of the brain reflect customary. The product of dopamine HVA in each group was not statistically significant. |
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