Medicine Eradication Of Helicobacter Pylori (inhibition Experiments)

Purpose:we optimized application by researching the Anti helicobacter pylori mixture of years clinical application which have indeed ideal effect. And using Uniform design method to launch in vitro. and then screened optimal combination. 12 traditional Chinese medicine have been streamlined for 5 traditional Chinese medicine . Using uniform design method to streamline square formula dose and do in vitro pharmacodynamics experiment. According to the result of the experiment elected first three combination. And discuss the mechanism of action.Material and method:1. Material: Micro aerobic constant temperature incubator,Super purifying bench,High-purity oxygen,High purity nitrogen,Carbon dioxide gas( Shenyang xin dongji gas Co., LTD ),Temperature humidity table (shenyang love's prachanda instrument Co., LTD.),H. pylori strains (Beijing CDC) Cerebral heart soaking liquid AGAR (Beijing huaye chemical Co., LTD.), and mixed antibiotic , vancomycin, trimethoprim-sulfamethoxazole thickening agent oxidase ZSO bacteria, urease (jie meigene microbial reagent Co., LTD), sheep serum. Chinese medicine: astragalus, dandelion, sophora flavescens, radix scutellariae, rhizoma coptidis2. Method :Cultivating and preserve helicobacter pylori. By 5 composed of traditional Chinese medicine with five factors and optimize formula level uniform design table design group. According to literature and clinical experience sure every flavour drug dose, preparation of liquids, TCM muskone experiment: antiseptic experiment, MIC (minimum bacteriostatic concentration) experiments and paper method (antimicrobial susceptibility test). Drug resistance reaction experiment. Medium PH of TCM compound MIC influence. The experimental result by uniform design software of statistical analysis, draw the regression equation, and select pharmacodynamic good first three combinations.Results:1. Antibacterial experiment: sterilization rate better respectively :9d groups: 89.5﹪, 8c groups: 85﹪, 6c groups: 82﹪2. MIC (minimum antibacterial concentrations) experiment: Chinese medicine prescription 6 group used alone, MIC80 helicobacter pylori 62.5ug/mL for:; TCM formula 8 group used alone; Helicobacter pylori for: MIC80 62.5 ug/mL Chinese medicine ninth9 group used alone, MIC80 helicobacter pylori for: 31.25 ug/mL. Combining microscopically of them, 6,8, 9 groups of TCM formula inhibiting bacterial growth effect is good3.Different combination drugs MIC measuring: TCM formula between the traditional Chinese medicine has synergy or add function. Prescription form a rationality, share can enhance efficacy.4. The determination of bacteriostatic circle around 41mm bacteriostatic circle, are: 6c, 8c, 9d, the9. Top three kinds of Chinese drug good respectively dose combination of 6, article 8 group, the 9th group.Conclusion:Through in vitro experiments screening resistance helicobacter pylori advantage combination: astragalus, dandelion, sophora flavescens, rhizoma coptidis, born Kennedy: : and best compatibility dosage: the first six groups is as follows: the dose of drug 8g, 15g, 20g, 10g, 2gb of 8 doses as follows: 24g, 12g, 28g, 2g 0g and 9 group, is as follows: the dose of drug 24g 32g, 32g, 16g, 8g confrontation, h. pylori does work.