| ABSTACTRheumatoid arthritis is a chronic disease mainly characterizedas peripheral symmetric and polyarticular inflammation, it alwayslasting long time and having high cripple rate. Neither the causenor the mechanism of RA is entirely clear. It has been known thatmonokines including IL- l 0 and TNF- Q secreted bysynoviocytes, chondrocytes, macrophage have a main effect in themany processes of RA, such as acute synovial inflammation,fibrosis, distruction of bone and cartilage, etc. As two kinds ofimportant inflammatory mediums, nitric oxide (NO) andprosglandin E 2 (PGE2) have a widely effect in the mechanism ofRA. So, it must be a reasonable way to treat RA by restrain thesynovial membrane secreting too many cytokines such as IL-l 6,TNF- Q or releasing too many inflammatory mediums such as NO,PGE2.Baihu Zhuifeng Wan (BFZFW) was created on the base ofBaihu JiaGuizhi Tang. It has the effects of clearing heat andremoving dampness, dispe1ling wind and activating channel,alleviating pain. lt has been proved having a good therapeuticeffect for early RA through clinic operations for many years. Tostudy the mechanism of this effect deeply, we observed thetherapeutic effect on adjuvant arthritis(AA) rats by BHZFW, andthe effect on the secretion level of IL-l 6,TNF- Q and releasinglevel of NO, PGE2 by primary cultured synoviocytes derived fromthose AA rats.The results of this discussion indicate that:1. Although we can get primary cultured synviocytes by bothdigesting method and tissue method, just digesting method makes itSure that we can observe three typical kinds of cell shapes:3Macrophagelike cells (MCs), fibroblastlike cells (FCs), dendriticcells (DCs). At the same time, we get only one kind of cell shapeby tissue method: FCs. The best condition for digestingsynoviocyte is digested by 0. l% coIIagenase for 2.5 hours, and thedigested by 0.25% tripsin for 0.5 hour.2.The therapeutic effects of BHZFW on AA rats.The middle and low dose of BHZFW obviously make the addedweight of AA rats larger than the control. High dose andtriterpenoids make the added weight smaller than the control. Suchresults showe a two way regulation effect to the weight adding ofAA rats. Each dose of BHZFW showe a good restraining effect onthe sweIling rate of AA rats' paws, especially the continuedsweIIing, but there is not obviousIy difference from the effect oftriterpenoids. There is a positive corre1ation between the pawvolume method and paw thickness method on measuring theswelling rate. Pathological study indicate that all the doses ofBHZFW can Iighten the synovium sweIling and inflammatory ceIlsinfiltration, high dose is more obviousIy. Triterpenoids also canlighten the infiltration of inflammatory cells but always aIong withenl arged .vessel s.3.The effect of BHZFW to primary synviocyte function.Each dose of BHZFW can decrease the IL-l or TNF- Q levelsecreted by primary cultured synviocytes derived'from AA rats, sodoes NO and PGE2. There is not any obvious difference betweenBHZFW and triterpenoids.Making BHZFW as treating factor and AA rats as tested object,we researched the anti-RA mechanism of BHZFW from the metl1odof cytopharmcological by primary cu1tured digested synoviocytesin this discussion. The resu1ts of this experiment showe that the4mechanism is concerned with decreased lL- 1 P, TNF- Q secretionlevel and NO, PGE2 releasing level by synoviocytes. In this articlewe also consider the seropharmcological experimental method asa usefuI approach tbr fLlture study. |
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