Igf-i On Hypoxic-ischemic Neonatal Rat Brain Cell Apoptosis

OBJECTIVE: To study the effects of exogenous Insulin Like Growth Factor-I (IGF-I) on cerebrocellular apoptosis of hypoxic-ischemic Newborn Rats and verify whether or not the IGF-I could pass through the blood-cerebral barrier. METHODS: The HIE model was made from 7-days newborn Wistar rats and different dosage of IGF-I were intraperitoneally injected into the abdominal cavity. The rats were killed after hypoxia and ischemia for 48 hours and the number of cerebrocellular apoptosis, apoptotic rate and apoptotic dense of rats were detected by HE and TUNEL stain, and the results were compared to among the salivia militirrhixae group, saline group, FIlE group and normal control group. RESULTS : The number of cerebrocellular apoptosis, apoptotic rate and apoptotic dense in HIE group were much higher than that in normal control group (P < 0.01), those in appropriate dose of IGF-I treatment group were much lower than that in FILE group and saline group (P < 0.01, P < 0.01), but there were no significant different between FILE group and salivia militirhixae group(P>0.05). CONLUSION: (1) There was cell apoptosis when the newborn rats with brain injury; (2) Exogenous IGF-I could pass through the blood-cerebral barrier, the appropriate dose of IGF-I has similar effects of the salivia militirrhixae on inhibiting cerebrocellular apoptosis and has the therapeutic effects for the I-HE.