Cultured In Vitro Endothelial Cell Adhesion Dynamics And Blood Components

A method for analysis of blood cells to the endothelial cells (ECs) based on the endothelial cell culture, microfluorescence technique was developed. Through a parallel plate flow chamber model and EC injury, the adhesion of platelets, polymorphonuclear leukocytes (PMN) and lymphocytes to the endothelial cells was successfully formed in vitro. By this method, We measured the parameters quantitatively such as platelet adhesion rate (coverage), white cell adhesion percentage after the reaction of endothelial cells and blood cells under the fluid shear stress condition and studied the anti-adhesive effect of some medicines.The whole system is consisted of parallel plate flow chamber model, Laser Scan Confocal Microscope (LSCM). This in vitro adhesion model and the corresponding quantitative analysis method is useful in the studies of interaction of endothelial cells with platelets and leuco-, and mechanism of thrombosis and atherogeniesis. It is expected to be applicable to evaluate the effects of antithrombus and anti-inflammation drugs.