Three Candidate Genes With Type 2 Diabetes In The Xinjiang Region Correlation Study

Type 2 diabetes mellitus is a clinically and genetically heterogeneous disorder. However, the majority of susceptive genes to the disease are still to be described.The aim of the study is to research the relationship between three candidate genes:glucagon receptor gene(GCG-R gene),insulin receptor gene (INS-R gene) and sulfonylurea receptor gene (SUR1 gene) with type 2 diabetes mellitus in Xinjiang. DNA were extracted from blood samples from 72 subjects, including 42 type 2 diabetes mellitus patients and 30 controls. PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) were used to detect the mutation .No mutation in exon 2 of GCG-R gene was detected in both groups. Five abnormal electrophoresis strands of exon 17 of INS-R gene were found in 42 type 2 diabetes mellitus patients while none was found in controls. The frequency of 揷?allele in intron 24 of SUR1 was 77.5% in patients and 63.5% in controls, and that of the 揷c~~ genotype was 6 1.9% and 40%, respectively. But none of the data showed signifcant difference The results suggested that the mutation in exon 2 of GCG-R gene and in intron 24 of SUR1 gene may not associate with type 2 diabetes mellitus in Xinjiang area, but the mutation of exon 17 of INS-R gene may relate to the disorder.