.919 Syrup Anti-rat Liver Fibrosis In Rats

Objective:We applied modern medical to design the experiment on the basis of the former accomplishment this time.The roles of 919 syrup in preventing and treating compounded-factor-induced Hepatic fibrosis were explored in order to provide theoretical and experimental basis for clinic.Method:72 Wistar rats were randomly divided into 6 groups.Each included 12 rats. The models were induced by complex factors of CCU and foods of high fat and high cholesterol and low protein.40%carbon tetrachloride and olive oil solution 5ml/kg the first day at experiment, was hypodermically injected to all rats except normal group. Since then, the dose was changed into 3ml/kg every other 3~4 days. All groups were treated by perfusing stomach while the rat began to be induced the model. The normal group and hepatic fibrosis model group were treated with sodium chloride lOml/kg everyday; The colchicines treatment group were treated with colchicines O.lmg/ml everyday; The large, middle and small dose 919 syrup treatment group were treated with 919 syrup 20ml/kg, 10ml/kg,5ml/kg espectively everyday. At the end of 6th week, all rats were hocused with 2% pentobarbitalum natricum and killed. The serum level of hyaluronic acid(HA), precollagen type IH(PCIII), laminin(LM)were detected with RIA. The liver tissue was studied with pathological observation. Hydroxyproline(Hyp)in liver tissue was detected with Xu ?s methods. All data were compared among the six groups.Results:1.The level of serum HA,LM,PC1II in rats of treatment groups significantly decreased,the effect has shown significant distinction by comparison with hepatic fibrosis model group ( PC0.01 ) ;There was no difference between the large dose 919 syrup group and the colchicines treatment group in the level of serum HA,LM,PCIHand Hyp( P>0.05 ); The effect of large dose 919 syrup group was better than the other 919 syrup groups ( P<0.01 ) .2. Optical injection, the denaturation and necrosis level of hepatocytes and the distribution of collagen fibrosis increased obviously after hypodermic injection of CCU for 6 weeks. Electron microscope, the shape and structure of cell organells were destroyed obviously. Fat storing cells and Kuppfer ?s cells increased. Collagen fibrosis distributed extensively in the model groups. After 6 weeks treated with 919 syrup, the pathological changes and the collagen fibrosis related with hepatic fibrosis above-mentioned decreased significantly.Conclusions:919 syrup has shown definite effect on anti-hepatic fibrosis.