| The peptic ulcer is one kind of common alimentary disease. In the traditional Chinese medicines, a lot of medicines are more effective for the peptic ulcer through recuperating the spleen and stomach. The powder for treating cold limbs (PTCL) which treated the stagnation of liver - qi , disturbance of qi, stagnation of yin and so on before comes from the 《Treatise on Febrile Disease》. There are four kinds of medicines-. Thorowax root, White peony root, Immature bitter orange and Licorice root. The thorowax root could relieve the depressed liver. The white peony root could enrich blood and astingting yin fluid and nourish the liver. The immature bitter orange could relieve stagnation of to remove food. The licorice root could invigorate the spleen and stomach. Recently some results of animal experience and clinical research explain PTCL is effective for the peptic ulcer, especially for the gastric ulcer. It was reported PTCL inhibit the output of gastric acid and increase the GMBF. The licorice root could inhibit the output of gastric acid and pepsin. The thorowax root and white peony root could prevent the stress - induced gastric ulcer. The immature bitter oranges effect for the peptic ulcer hasnt been reported. In this recipe, there are major medicines and minor medicines and there are sole effects and synthetic effects. In order to understand the effects and interaction of every medicine of PTCL for the gastric uleer , we took the gastric acid , gastric pepsin and ulcer length as the target and analyzed the effects of every medicines and different combinations of four kinds of medicines with the orthogonal design. The orthogonal design is onekind of the scientific statistical method, which could arrange the more factors and compare with every factor and analyze the interactions and help us find the best combination of the recipe.ObjectivesThe present study was to investigate the effects of PTCL and the different combinations of PTCL s four kinds of medicines and interactions of four kinds of medicines for the output of gastric acid and pepsin and the length of gastric ulcer with the orthogonal design through producing the animal models.Materials and MethodsMaterials; Male Wistar rats. Drugs: Thorowax root(5g) ,White peony root(4g) , Immature bitter orange (2g) and Licorice root (1.5 g). It was grouped based on the table of orthogonal design { L16 (215) }. Every group of drugs was boiled for 30 min by gentle fire after soaked in the distilled water 350 ml and the medicine solution was took into the dry fine powder by dryness system . The dry fine powder was placed in the surroundings of 4t. Instruments: the spectropho-tometer ( UV - 260 ) , the appliances for operation, the syringe for stomach, syringes, the tubes, the water bath box , the burets, the scale. Reagent: Hb of cattle, L-tyrosine, Folin reagent,0. 3N,0.2N HC1,0.01N,0.5N NaOH,0.3N trichloracetic acid. Methods: The rats were grouped randomly. Every group had 16 rats. Every group of dry fine powder was dissolved in the 20ml of distilled water. Drugs (20ml/kg) were given 10 min before water immersion. The stomachwas removed 7 h after water immersion. At last, the ulcer was confirmed and measured. During producing the acetylsalicylic acid (ASA) -induced gastric ulcer, drugs(20ml/kg) were given immediately after pylorus ligature and the gastric juice was collected and analyzed for its acidity and pepsin output 7 h later. Data was analyzed by the direct analysis and variance analysis.ResultsOutput of gastric acid; The licorice root markedly inhibited the gastric acidity output( p <0. 01) . The thorowax root, PTCL and PTCL without white peony root inhibited the gastric acidity output ( p < 0.05 ). White peony root enhanced the gastric acidity output. The immature bitter orange hadnt marked difference (p >0.05) .Although the licorice root and thorowax root inhibited the gastric acidity output, the combination of both hadnt marked difference (p > 0.05). It explained the interaction of both was negative effect. If t... |
PDF Full Text Request