| Objective:To study the preventive and therapeutic effect of Tangweikang (TWK), a preparation of traditional Chinese medicine (TCM), to the Diabetic Gastroparesis (DGP), and explore the mechanism and offer the scientific basis for TWK's clinic application and exploitation. Methods:48 Wister diabetic rats were randomly divided into four groups: model group, Paspertin treatment group, TWK low dose treatment group, TWK high dose treatment group, 12 rats in each group. The diabetic rats were induced by IP of STZ, 60mg/kg and their blood sugar were continue higher than 16.65mmol/L.Further more choose another 12 healthy rats as the control group. The control group was feed regularly on standard rats feed. The other four groups were fed on high calorie feed (high sugar and fat) irregularly, eating in odd-number days and fasting in even-numbered days. All the rats drunk water freely. During the experiment period, the appetite, water volume, urine volume, stool character should be observed, and weight the rats, test the urine sugar once every week. Each group was perfused stomach with drugs during establishing the model. The control group and the model group were perfused stomach with saline, lOml/kg. TWK high dose treatment group 1 Oral/kg (23.8g/kg). TWK low dose treatment group 15ml/kg (5.95g/kg) and WFA treatment group 10ml/kg (3.4mg/kg), once per day, lasted 4 weeks. Recorded the weight and the blood sugar on an un-empty stomach. The day before the last day, Fasting 24h after the last feeding, each group fed carbo-powde suspension by orally taking (activated carbon and acacia each contains 10%), 10ml/kg.put to death after 30 minutes. Calculating the stomach-intestine driving marker. The pyloric part should be put into 10% formalin solution, after routine desiccation, hyaline dipping in wax fixated and embedding, then making VIP and CGRP nervous immunohistochemistry mark, doing the image analysis, calculating the area and light density of the VIP and CGRP nervous in the pyloric part. Result:3 days after establishing the models, rats of the model group and the treatment group got drink and eat more, urine volume increased, two weeks later the rats got to be with poor appetite, lassitude and inertia, less stool, losing weight obviously than the control group.4 weeks later, the rats of the model group were worse than the control group, emaciation, lazy to move, poor appetite, no aggressiveness and easy infection, survival rate sharply decrease (7/12); the rats of the model group compared with the control group, blood sugar significantly increased(P<0.01),weight obviously decreased,stomach-intestin driving marker sharply decreased (P<0.01) ,image analysis shows that the VIP and CGRP nervous' area and light density of pyloric part obviously decreased (P<0.01),each drug treatment group compared with the model group, the survival rate is respectively as folio wed :Paspertin treatment group is 8/12,low and high dose TWK treatment groups are 8/12 and 9/12.the blood sugar decreased than the model group(P<0.05),weight increased, the high dose TWKtreatment group compared with the model group(P<0.05);as the stomach-intestine driving marker was concerned, Paspertin treatment group and TWK high dose treatment group increased obviously than the model group(P<0.01),there is significant difference between the TWK low dose treatment group and the model group(P<0.05),as the VIP and CGRP nervous' area and light density was concerned, the TWK high dose treatment group significantly increased than the model group(P<0.01),the TWK low dose treatment group increased compared with the model group(P<0.05),there is no difference between the TWK high dose treatment group and Paspertin treatment group, on the stomach-intestine driving marker(P>0.05),there is significant difference on the VIP and CGRP nervous' area and light density (P<0.01),there is difference on the stomach-intestine driving marker,VIP and CGRP nervous' area, light density between the high and low dose of TWK treatment group, it states that TWK can improve the pe... |
PDF Full Text Request