Tongbi I Treatment Of Rheumatoid Arthritis, Experimental Research And Clinical Observation

In this experiment, we inject the CFA to the big rats to build AA rat model to simulate human beings Rheumatic arthritis, and setup the Tong-bi-I liquid in high, mid, low dose groups and leigongteng contradistinguish group and blank group. Observe and handle with statistics the levels of IL-6 and thymus gland, spleen, adrenal gland wet weighs of big rats in AA model, at the same time make joint synovium pathology sections, then evaluate the influence of the Tong-bi-I in immunity in big rat in AA and as to their joint synovium, investigate the immunology mechanism of that medicine treat rheumatism arthritis, Observes the maximal tolerance dose of the small rats to assess the safety of Tong-bi-I liquid. Through clinic observe to evaluate the effect of Tong-bi-I. MethodMake 40 big white big rats in building the model all, the successful big rat models are divided into 5 groups at random, named the group of A, blank group, gives physiology brine; The group of B, low density in Tong-bi-I group, gives 67% Tong-bi-I; The group of C, Tong-bi-I mid density group, gives 134% Tong-bi-I; The group of D, high density in Tong-bi-I group, gives 268% Tong-bi-I; The group of E, leigongteng group, gives 5mg/2ml leigongteng liquid. Begin to give the medicine after 19 days form making model, each rats only give medicine by the 20 mis/Kg calculation, everyday give medicine once, giving medicine time is 7 days. Observe each animal joint swell degree and skin bottom knot stanza circumstance, weight and an etc circumstance. Observe and handle with statistics the levels of IL-6 and thymus gland, spleen, adrenal gland wet weighs of big rats in AA model, make joint synovium pathology sections.20 Small rats are not divided into groups, they are given 268% Tong-bi-I for 10days,three times each day. Observe small rats action and death.48 RA patients are given Tong-bi-I common dose for 3 months, observe their joint swell, pain, function and ESR, ASO, RF etc. ResultThe B, C, D, E groups all can obviously alleviate the weights of spleen , thymus gland, adrenal glands and the IL-6 levels of the big rats. Compare with group A, P<0.05. There have no obvious difference among C, D, E groups. The B, C, D, E groups all can obviously alleviate the rats joint pathology changes. Among 10days,except some rats stool become watery, there has no dead small rat. 3 months therapy does great good to the joint swell, pain, function and ESR, ASO, RF of the patients. ConclusionTong-bi-I can repress the immunity function and the pathology change in the joint of big rat in AA, the immunity represses function of high and mid dose group are equal to that of leigongteng product.Tong-bi-I is a kind of safe medicine.Tong-bi-I is a valid medicine of RA.