Study Of Of Aconite Chemical Composition And Active Ingredients

Aconitum carmichaeli Debx. is a kind of Chinese traditional medicine in common use. The task which was assigned by Nation Science and Technology Department,would research ten kinds of important Chinese traditional medicine . Aconitum carmichaeli Debx. was one of them. It will accelerate the development of Chinese traditional medicine, quicken Chinese traditional medicine modernization. This paper, which based on a great deal of chemical and pharmacological literatures in the world, researched the chemical components , pharmacological effect, standard compound and fingerprint chart. The results indicated:1 Separation four kinds of chemical componentsThe important chemical components are alkaloid in Aconitum carmichaeli Debx.. This research separated 15-α -OH neoline, aconine, β-sitosterol and uracil from Aconitum carmichaeli Debx..It is the first time separated 15-α -OH neoline from Aconitum carmichaeli Debx..with High Speed Counter Current Chromatogram.2 Identification the structures of four kinds of chemical componentsBy the way of physical-chemical method ,TLC,UV,IR,MS and NMR, the structures of four kinds of chemical components which were separated in this research were identified.3 Preparation and accumulation some quantitive standardsUracil was selected as the standard ,which based on the research of chemical components. 1200mg uracil with the purity of 100% proved by HPLC was got.4 Founding the method of quantitative determination of uracil in Aconitum carmichaeli Debx.Founded the method of quantitative determination of uracil in Aconitum carmichaeli Debx. by RP-HPLC for the first time. Determined the content of uracil in Aconitum carmichaeli Debx. which were preparated by different methods.5 Clarification the pharmacological effect of uracilThe pharmacological research indicated that uracil is one of the enhanced-heart components.6 Seting up the fingerprint chart of Aconitum carmichaeli Debx.The research about the fingerprint chart of Aconitum carmichaeli Debx., as the important content of Chinese traditional medicine modernization, was carried out for the first time. The fingerprint chart and inspection standard were established.