In Vitro Cultivation The Bezoar Polyacrylamide Gel Electrophoresis, And The Study Of The Bezoar Qianliekang Tied Pharmacy Part,

Invitro Cultured Calculus Bovis is collected in the standard of national drugs and it is the substitute of natural Calculus Bovis.it is a widely-used traditional Chinese medicine for anti-inflammatory,detoxifiry and liver-defence.Compared with natural Calculus Bovis, Invitro Cultured Calculus Bovis has the same chemistry . In additional , it schief chemistry are not only more than natural Calculus Bovis, bue also less lost than natural Calculus Bovis;compared with man-made Calculus Bovis, it has better quality and better effect.The thesis established the research methods of proteins of Invitro Cultured Calculus Bovis by the way of IFE,SDS,PAGE etc. we defined the Isoelectric Focusing lilectyophoresis and the number of moleoular . Besides we can identify Invitro Cultured Calculus Bovis by the research of PAGE.Niuhuang Qianlie kang Suppository is carried out the research on its preparation technology quality standard and primary stability . The results apply reliable base for the preparing and clinic effect of Bile acid Berberine, Baicalein Niuhuang Qianlie kang Suppository.We selected total quantity of dry ointment and Baicalin as marker , utilizing L9(34) orthogonal Test to optimize the process of ethanol-extraction and determined it as following:decocted twice, first six times volume 70%ethanol 2. 5hr and then four times volume 70% ethanol 1. 5hr concentrated to 1. 15-1. 20g/ml(80 ℃). In order to reduce the producing perods, we select spray-drying methods to desiccate this ointment, air-inlet temperature at 150*C, air-outlet temperature at 95℃-100℃ and pressure 0.7Mpa in drying tower .During the quality research proceedings. We indenti fied Bile acid Berberine, Baicalein etc by T1,C (Thin Layer Chromatograph)method, which is soccial and accurate. The content of Baicalin and bilirubin is assayed by HPLC(High performance Liquid Chroma tograph) after the methodology was completed. Forthe sake of insoecting primary stability of Niuhunag Qianlie Kang suppository , the room-tempeerature test was carried into execution, from which the results showed every index marker was noral and consistent, which indicated the quality of Niuhuang Qianlie Kang Suppository was steady and controllable.To sum up , the pharmic study elucidated that, on this product , its preparation technology was reasonable, quality standard definite and stability perfect.