Hepatitis B Patients Infected With Hbv Serum Markers Of Quantitative Research And Its Clinical Significance

1.2.1 Objective:l.To detect quarititation of HBV markers in serum and to study their clinical sigriifiranee . 2. To study the relationship of HBV DNA and HBV markers in serum . 3. To study HBV DNA distribution in different group . To provide test evidences for early diagnose> to guide usingdrug and to observe curative effect of patients infected by HBV.1.2.2 Methods:1. Time-resolved fluoroimmune assay(TRFIA) was used to measure the quantitation of HBV markers in serum. 2. Fluoroimmune quantitative PCR(FQ-PCR) was used to measure HBV DNA. 3. Enzyme Linked immunosorbent assay was used to detect PreSl.1.2.3 Results:There is a direct correlation between quantitat ion of HBV DNA and HBeAg or HBsAg. There is a negative correlation between quariti tat ion of HBV DNA and HBcAb. Mean copies of HBV DNA is higher in the group of HBeAg(+) than the group of HBeAb (+). The positive rate of PreSl is higher in the group of HBeAg(+) than the group of HBeAb (+). Infect proportion is higher in the group of male than the group of female . It is higher in the group who were 20 to 50years old than the other two groups, too. The HBV DNA copy was inhigh or middle level in the patients who were in the period of immune tolerance , and it was in middle level in the patients who were in the period of immune deletion, and it was in low level in the patients who were in the period of virus remains, too. 1.2. 4 Conclusion:There is a following relationship between HBsAg and HBV DNA . They are the markers of virus duplication. HBsAg and PreSl are the ingredient of HBV surface. There is a direct correlation between their high"producing and HBV DNA duplication. Infect rate is higher in the group of male sex than the group of female sex and it is higher in the group of middle age and young than the group of early youth and old people. After people infected by HBV, there is not a correlation between the load of HBV DNA and sex or age . Using the two methods of TRFIA and FQ-PCR to detect for the infection of HBV have a very significance for the earlier diagnosis of HBV infection. Monitor the infection and effection of the drug in the clinic.