The Toremifene Sensitizing Effect Of Chemotherapy In Human Bladder Carcinoma Cell Line T-24,

Object:To evaluate the possible chemosensitization effects of toremifenecombined with anticancer agents(Adriamycin is selected) on human bladdercarcinoma cell line T-24, to explore its possible mechanism and provide newtherapy method for chemotherapy in bladder carcinoma. Method:The level of cell proliferation was measured by the MTT method,cell cycle was measured by flow cytometry with PI Staining (FCM) method,and the level of TGFβ-1 was measured with ELISA. Result:Level of proliferation of high-dose TOR-treated (≥20μmol/L) T-24cells decreased significantly compared with not-treated group(p<0.05). 5,10μmol/L TOR enhanced T-24 cells growth inhibition effect made by ADM, andmade IC50 decrease significantly ( p<0.05 ) . The potentiation of thecombinations was 1.26 and 2.7 individually. 5μmol/L TOR augmentedapoptosis of T-24 cells induced by ADM, made PI decreased significantly, andthe rate of TOR+ADM-treated T-24 cells in G0/G1 phase increased. TORenhanced production of TGFβ-1 and this action had dependenceon concentration of TOR . Conclusion : In vitro, 5 , 10μmol/L TOR can remarkably enhancechemosensitivity of ADM to T-24 cells. TOR can augmente apoptosis of T-24cells induced by ADM, increase the rate of T-24 cells in G0/G1 phase. TOR canenhance production of TGFβ-1. The possible mechanism of chemosensitizationeffect caused by TOR is associated with up-modulation of TGFβ-1 productionand prevention of G0/G1→S phase transformation.