| OBJECTIVE: In order to study the reverse effect of antisense oligodeoxynucleotide on tumor MDR in vitro in the future, we plan to establish human multidrug-resistant gastric glandular carcinoma cell line (SGC7901/ADM) and to investigate its biological characteristics. METHODS: An adriamycin-resistant human gastric glandular carcinoma cell line (SGC7901/ADM) was established in vitro by gradually increased concentration of adriamycin(ADM) in the culture medium. The cell growth was measured; the multidrug resistance to multianticancer agents of the cells was evaluated by MTT assay; the cell cycle and the intracellular mean fluorescence intensity of rhodamine123 were determined by flowcytometry (FCM) also. The expression of P-glycoprotein (P-gp), multidrug resistance-related protein (MRP) was assessed respectively by immunocytochemistry staining. RESULTS: Compared to SGC7901, SGC7901/ADM had a prolonged doubling time of 30.1h. The number of cells in S-phase was significantly decreased 22.5% in SGC7901/ADM while those in G1 and G2-phase increased 12.9%, 9.8%, respectively. Furthermore, SGC7901/ADM resisted to many anti-tumor agents, and its IC50 of ADM was 25 times higher than the parent SGC7901. Significant overexpression of P-gp and MRP of the multidrug resistant cells were detected. The intracellular mean fluorescence intensity of rhodamine 123 was decreased in SGC7901/ADM. CONCLUSION: SGC7901/ADM is a human multidrug-resistant cell line, and it's drug resistance related to the overexpression of P-gp and MRP. It is qualified to further research on multidrug reversing . |
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