Aconitum Extract Transdermal Absorption Mechanism

Transderm Delivery System(TDS) is also called Transdermal Therapeutic System(TTS). It refers to a kind of preparation. Drug in this preparation can get through skin, be absorbed by capillary vessel and then go into systemic circulation and take effect. Preparation is absorbed mainly by going though cuticle and epidermis, soaking dermis and falling into systemic circulation by capillary vessel. As far as most of medicines are concerned, skin is a barrier for transderm delivering. For a lot of medicines, the penetrating rate falls short of their treatment demands. So finding the way to promote drug absorption is the key to developing Transderm Delivery System. It includes physical, chemical and pharmacy methods. Penetration enhancers can raise the speed of transderm delivering or increase the amount of drug penetrating. It's mostly researched as a method to promote the penetrating absorption of drug.Iontophoresis(IP) is to properly apply direct current on skin to advance the process in which drug molecule penetrates skin and get into organism. Drug cations penetrate skin at anode and drug anions do at cathode. Neutral molecule can also get through skin under the function of electroosmosis. By iontophoresis, we can promote effectively the penetrating absorption of drug especially ion-type drugs and polypeptide large molecules. And the role of electric field enhances water's passing by skin, so the penetrating of non-electrolyte drug is also been advanced.Cuticle is composed of cutin cells rich in lipid and only has 20% water content. So it's non-conductor of electricity. When currents go through, cuticle produces impedance. When direct current is used continually, charges are stored and produce polarized currents with the opposite direction of DC currents. The polarized currents apparently repel drug ion's moving to opposite electrode and reduce the efficiency of iontophoresis. If we use pulse currents, choose appropriate waveform, frequency and duty ratio,skin polarization phenomenon will probably be removed.The aim of this research is finding out the best conditions and methods to promote the penetrating absorption of drug by observing the effect of different promoting penetration ways on the rate of meaconitine kusnezoff monkshood root extractive of velocity the influence of external extractive of kusnezoff monkshood root on animal physiological indicators under these different methods. By doing this, we hope we can develop new medicaments.This research consist five parts: distilling active ingredients from kusnezoff monkshood root, menstruating mesaconitine content of extractive of kusnezoff monkshood root, determining penetrating rate of mesaconitine, the influence of outside using extractive of kusnezoff monkshood root on engorgement in rat's voix pedis and observing morphology of animal skin Organization.During the first part of the experiment, we distill ingredients from kusnezoff monkshood...