Huang Jinju Preparation Of Quality Assessment And Identification Of Biological Effects Is Discussed

1 AimTo establish the standard of quality of Huang jin ju Buccal Tablet, Huang jin ju Capsule. Try to find a method to control the quality of Huang jin ju powder for injection by determining the effect of resisting virus in vitro.2 Content and Method2.1 To develop a method to determine Huang jin ju Buccal Tablet, Huang jin ju Capsule2.1.1 To establish product process of Huang jin ju Buccal Tablet, Huang jin ju Capsule,For the Buccal Tablet find a sound proportion of ethanol consistence and additive by studying the result of making pellet and tablet.For the Capsule find a sound proportion of ethanol consistence and additive by studying the result of making pellet.2.1.2 To establish a standard of quality Huang jin ju Buccal Tablet, Huang jin ju CapsuleTo establish a method to determine the content of Orientin, Baicalin, Chlorogenic acid in Huang jin ju Buccal Tablet, Huang jin ju Capsule by HPLC.2.1.3 The pharmacodynamics of Huang jin ju CapsuleThe aim is to provide a direction to establish a biological identification method to control the quality of Huang jin ju Capsule. The content are anti-inflammatory, abirritation, antitussive, febrifuge, antibacterial.2.2 Find a biological method to evaluate the Chinese medicine quality related to the treatment affection.The research carrier is Huang jin ju powder for injection with more refined, more known component and less additive than the others Huang jin ju preparation, and it is the emphases in all research of Huang jin ju preparation.Using the cell culture technology in vitro, establish the method to determine the effect of Huang jin ju powder for injection resist the Ad3. Using the chick embryo technology, establish the method to determine the effect of Huang jin ju powder for injection resist the influenza virus PR8.3 Results3.1 Establish the standard of quality of Huang jin ju Buccal Tablet, Huang jin ju Capsule3.1.1 Establish the product process of Huang jin ju Buccal Tablet, Huang jin ju Capsule3.1.2 Establish the quality standard of Huang jin ju Buccal Tablet, Huang jin ju Capsule by HPLC.3.1.2.1 The quality standard of Huang jin ju Buccal TabletDetermining Orientin: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.1% H3PO4 water: acetonitrile (83:17) at a flow velocity of 1.0mL /minute; the detecting wavelength was 345 nm, the average recovery was 99.97%, RSD=0.8217 %.Determining Baicalin: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.055% H3PO4 water: methanol: tetrahydrofuran (53.9:38.2:7.9) at a flow velocity of 1.0mL /minute; the detecting wavelength was 280 nm, the average recovery was 95.97%, RSD=1.06%.Determining Chlorogenic acid: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.4% H3PO4 water: acetonitrile (89:11) at a flow velocity of 1.5mL /minute; the detecting wavelength was 327nm, the average recovery was 95.97%, RSD=1.06 %.3.1.2.2 The quality standard of Huang jin ju CapsuleDetermining Orientin: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.1% H3PO4 water: acetonitrile (83:17) at a flow velocity of 1.0mL /minute; the detecting wavelength was 345 nm, the average recovery was 100.87%, RSD=1.4746%.Determining Baicalin: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.055% H3PO4 water: methanol: tetrahydrofuran (53.9:38.2:7.9) at a flow velocity of 1.0mL /minute; the detecting wavelength was 280 nm, the average recovery was 100.44%, RSD=0.5389%.Determining Chlorogenic acid: Using ODS2 C18 4.6×250mm, 5μm column, the mobile phase was 0.4% H3PO4 water: acetonitrile (89:11) at a flow velocity of 1.5mL /minute; the detecting wavelength was 327nm, the average recovery was 100.44%, RSD=2.94%.3.1.3 The result of the pharmacodynamics of Huang jin ju Capsule The results indicate Huang jin ju Capsule has the effect of anti-inflammatory, abirritation, antitussive, febrifuge, antibacterial, and may try to establish a biological identification method. 3.2 Research identification methodology by Huang jin ju powder for injection resisting virus in vitro.Establish the quality standard of Huang jin ju powder for injection resisting virus in vitro.3.2.1 the lowest effect concentration of resisting Ad3 virus is 25mg/mL.3.2.2 the lowest effect concentration of resisting influenza virus PR8 is 6.25mg/mL.3.2.3 the highest safety concentration to hela cell is 100mg/mL3.2.4 the highest safety concentration to chick embryo is 100mg/mL4 Conclusions4.1 Find out the optimal product process.4.2 Establish a quality standard to determine the content of Orientin, Baicalin, Chlorogenic acid by HPLC.4.3 Find the lowest effect concentration of resisting Ad3 virus and influenza virus PR8. Find the highest safety concentration to hela cell and chick embryo.4.4 Establish the method of biological identification Chinese medicine by determine the activity of resisting virus. Find a new method to evaluate the quality of Chinese medicine.