The Chemical Composition Of Loquat

The loquat leaf which is the leaf of Eriobotrya japonica( Thunb .) Lindl as a common traditional Chinese medicine,is recorded in clinical application of many medical books.It contains many penta-collar triterpenoid compounds and the main active composition is Ursolic acid.The study of contemporary pharmacology shows that Ursolic acid has hepatoprotective effects,anti-tumor effects,anti-inflammatory effects and anti-hyperlipidemic effects.and it has important medicine-used development.So we build up a method of HPLC to analyse the containing of Ursolic acidThe first step of the topic is to produced 20 kilo dry loquat leaf ,and then use medical crushing machine to make leaf pieces,and chose ethanol as percolating solvent. To concentrate percolating solvent and recover ethanol,the producing is black-green extract.Then chose ligarine, acetic ether and n-butanol in order to abstraction.According discern using 200-300 pillar,the part of acetic ether and n-butanol extrat is discern purification.After crystalling and recrysting several times,I obtained four chemical compounds. After that,we built up a method of HPLC to analyse the containing of Ursolic acid which is very important in medical research.The topic also extract volatile oil from loquat leaf two of different methods,and built up a method of GC-MS to analyse the containing of volatile oil...