Investigate Yang Wu Tang Influence And Mechanism Of Interstitial Fibrosis In Experimental Rat Lung Tumor Necrosis Factor-alpha (tnf-alpha)

Objective: To evaluate the therapeutic effect and mechanism of Buyanghuanwu decoction(BYHUD) in treating pulmonary interstitial fibrosis (PIF) induced by bleomycin in rats.Method: PIF models of bleomycin-induced pulmonary fibrosis were established in rats. 130 SD rats were randomly divided into five groups, including the normal group, the modeled group, the Metreton group, the group I and group Ⅱ of BYHUD . Expect the normal group had 10 rats, other groups had 30 rats respectively. Then the rats were treated with gastric infusion of normal saline, metreton or BYHUD respectively after 24 hours. Both group were put to death on the 7th, 14th , 28th day except the normal group ,which only put to death on the 28th day. The pathological changes, hydroxyprolin (HYP) level, Tumor necrosis factor (TNF-α ) of the lung tissue were measured. Bronchoalveolar lavage were collects and ELISA was applied for TNF- α quantitation.Results: BYHUD can inhibit abnormal heighten of hydroxyproline(HYP), Tumor necrosis factor (TNF-α ) content. Amelioration the pulmonary alveolitis and fibrosis were shown after treatment by BYHUD.Conclusion:BYHUD has definite effect in treating PIF. The mechanism may be through inhibiting the Tumor necrosis factor protein, decreasing collagen protein, so as to ameliorate the inflammation and fibrosis.