The Preparation Of The Soy Isoflavone Composition And Biosynthesis Of Initial Research

Isoflavone composition was prepared by extraction in organic solvent and purification with column chromatography.(macroporous resin, aluminum oxide and silica gel). Three purified compounds obtained from the soybean after two procedure of HPLC preparation were identified by spectral analysis, and they were daidzin, glycitin and genistin. The preparation method for Isoflavone composition was studied. It was found that the contents of daidzin, glycitin and genistin was added to more than 95% of all the purifications through the determination of HPLC.The method for the determination of isoflavone was established and evaluated. The results came to the conclusion that the method was suitable for the determination of isoflavone. The isoflavone in purified isoflavone composition, different kinds of beans and soybean tissues in different period of growth was determined.Based on the data of known isoflavone synthase 1, the specific primers were designed. The total RNA was extracted from the soybean seedlings. The core sequence (1127bp) of isoflavone synthase 1 cDNA was cloned with RT-PCR method, which was totally the same with the part of our expected target.