Electric Experimental Cerebral Ischemia And Reperfusion Injury In Rats Pain Threshold, Il-1¦Â, Of Enos, Nnos Of Ngf Effects Research

The cerebrovascular disease by its high disease incidence rate, the high mortality rate, cripples rate the severely impair humanity's health and the life high, speeds up along with the current economic condition improvement and the social development speed, people's rhythm of life and the food habit and so on has had the radical change, causes this sickness the disease incidence rate to increase unceasingly, the serious influence people's life quality, has created the serious family and society's burden. The acupuncture treatment cerebrovascular disease has a more ideal curative effect, by clinical widespread application. The acupuncture can improve the brain to lack the blood to pour into the damage animal model again many targets, the adjustment organism condition, plays the brain protective function, reduces the extent of damage, the promotion restores with the function improvement. This experiment observed the big mouse threshold of feeling change, the brain damage area pathology shape, the bast nitrogen monoxide to gather enzyme (eNOS), the nervous nitrogen monoxide gathered enzyme (nNOS), nerve growth factor (NGF) and circumference blood Bai Jiesu - 1β(IL-1β) the change and the acupuncture influence, confirmed the acupuncture from many angles to lack the blood to the experimental brain to pour into again damages the big mouse's therapeutic action.Objective: partial middle cerebral artery occlusion (MCAO) damages the pathological change which the big rat brain organizes, around the big rat brain damage the threshold of feeling change, returns to in the circle blood Bai Jiesu - 1β(IL-1β) the content change; Brain organization nerve growth factor (NGF), the bast nitrogen monoxide gathers enzyme (eNOS), the nervous nitrogen monoxide to gather enzyme (nNOS) the expression situation and the piquada"the paralysis of upper extremities","the latter three miles"the hole, to above various targets' influence, the research acupuncture treatment lacks the blood - pours into the damage again the overall function control action after the big mouse brain, and discusses the acupuncture control action the mechanism, to further deepen regarding the acupunture therapy anemic stroke action mechanism's understanding provides the new mentality and the basis. Methods: (1) the hitch long-base method unenlightened big mouse MCAO model; (2) the piquada"the paralysis of upper extremities","the latter three miles"the hole takes the treatment method; (3) uses the cold stimulation the method to observe the big mouse threshold of feeling the change; (4) HE dyeing observation lacks the blood side brain organization neuron to lack the blood necrosis situation; (5) emits in the immunity analytic method examination blood serum IL-1βcontent change; (6) the immunity group's method observes the big mouse brain organization to lack blood damage area NGF, eNOS, the nNOS expression change.Results: (1) early time in partial middle cerebral artery occlusion (MCAO) demage (1-24 hour) to lack the blood cerebral cortex again to present the neuron denatured necrosis, the brain damage and the degree progresses along with the time aggravates, the piquada may reduce lacks the blood area the extent of damage; (after 2) the brain lacks the blood pours into again, the big mouse's threshold of feeling to reduce, but is not big along with the time change threshold of feeling change, after the acupuncture may enhance the big mouse brain damage threshold of feeling; (after 3) the brain lacks the blood pours into the damage, again the circumference to return to the circle blood IL-1 expression standard namely obvious markup, the piquada may reduce in the circumference blood IL-1βthe content; (4) the brain lacks the blood to pour into again damages the big mouse brain damage area NGF expression upward, but has along with the time extension expression quantity reduces, the acupuncture may strengthen brain damage area NGF the expression, and may NGF continue maintains at the high expression condition; (after 5) the brain lacks the blood pours into the damage again, the NOS two kind of different hypotype nerve nitrogen monoxide and the enzyme (nNOS), the bast nitrogen monoxide and the enzyme (eNOS) has the expression, the acupuncture may cause nNOS to express weakens, eNOS expression enhancement.Conclusion: (1) early time in partial middle cerebral artery occlusion (MCAO) (1-24h) to lack pathology signs again and so on blood area cerebral cortex obvious neuron distortion necrosis, the acupuncture can reduce lacks the blood area extent of damage, plays the brain protective function; (2), in the brain lacks the blood to pour into around again the damage, the big mouse threshold of feeling has the change, the piquada may strengthen the big mouse to endure the degree to the pain stimulation, enhances the big mouse to the outside stimulation adaptiveness; (3) the brain damage area obvious nerve growth factor (NGF) the masculine cell, the acupuncture may strengthen its expression, thus promotion brain protection and repair function; (4) the brain lacks the blood to pour into the damage circumference to return to in again the circle blood IL-1βthe expression standard namely obvious markup, indicated that the brain lacked the blood to pour into again triggers the inflammation response, the piquada has been possible to reduce IL-1βin the circumference blood's content, reduced the inflammation damage; (5) IL-1βthe expression quite obvious phase, namely when damages 6h obviously elevates, 12 hours achieve the peak, when 24h have reduce; (6) the brain damage area may be simultaneously bright after the nitrogen monoxide gathers the enzyme (nNOS) and the bast nitrogen monoxide gathers the enzyme (eNOS) the masculine cell existence, the two in the brain lacks the blood to pour into the damage to display the different effect again, the acupuncture may cause the display brain protective function the eNOS expression upward, but causes the display nerve poisonous function the nNOS expression to decline, thus display brain protective function; (7) the eNOS expression has along with the time expand reduces, the acupuncture may cause eNOS to maintain the high standard.