Ann Palace Qingkailing Mechanism Of Inhibition Of Rat Brain Edema After Ich

This article includes review and experimentation.The former includes some inducement of the cerebral edema after intracerebral hemorrhage(ICH) and the research of chinese traditional medicine toward ICH.The latter includes three parts. The first mainly refers to the research of the pathological mechanism, using collagenase to induce the ICH in rat and observing the expression of iNOS and MMP-2 after ICH 24hours, 48hours, 72hours and 7 days, through these, to discuss the formation reason of cerebral edema. The second mainly refers to pharmacodynamics, through injecting Angong Qingkailing to cure the ICH rat , and using the measurement of biochemistry, molecular biology to observe these causes of cerebral edema.The third mainly refers to morphological research, through HE staining to analyse and compare the changes of the cerebral tissue in normal group ,model group, control group and Angong Qingkailing group , in addition , using immunohistochemistry to analyse the expressing location of VEGF and MMP-2.Results as follow:1. MMP-2,MMP-9 articipate the formation of the cerebral edema after ICHAccording to the damage of blood brain barrier(BBB), the formation of the cerebral edema after ICH can be categorized to the cerebral edema of blood vessel source and the cerebral edema of cell toxicity. the damage of BBB take part in the cerebral edema of blood vessel source, current research considers the formation of this cerebral edema having many reasons, including the damage of the free radical, cytokine, the active substance of blood vessel and NO ,the opening of the Ca2+pathway,and so on, especially the over-expression of MMP-2 and MMP-9. Abilleira finds that the ICH patients emerge nerve symptom in 24hours , simultaneously, the expression of MMP-9 increases, which relates to the cerebral edema surrounding the hematoma.My experimentation finds that the expression of MMP-9 gets climax in 24hours after ICH , this result is consistent with the the research of the overseas, and hints that the over-expressing of MMP-9 may be the cause of the cerebral edema of blood vessel source.The early research of the overseas considers that the expression of MMP-2 is different from the the expression of MMP-9,which begins in 24hours, gets climax in 5days ,and can maintain about 2 weeks, this refers that macrophage participate the course of repairment.Through ELISA detecting MMP-2 in serum, my experimentation finds that the over-expression of MMP-2 does not appear in 24hours ,but occurs in 48 and 72hours ,and slightly descents in 7days, which meets the research of the overseas.My experimentation finds that the low-dose Angong Qingkailing can comparatively inhibits the expression of MMP-9 , the middle-dose Angong Qingkailing can strikingly inhibits the expression of MMP-9, and the high -dose Angong Qingkailing and the Qingkailing can not inhibits the expression of MMP-9 in 48hours. Correspondingly, Angong Qingkailing and Qingkailing can not inhibits the expression of MMP-2,MMP-9 in 7days.2. iNOS articipates the formation of the cerebral edema after ICHAs the rate-limiting enzyme of NO ,iNOS can over-expresses after ICH, and then produces the toxic NO,leads to the death of the neuron, aggravates cerebral edema, so the detection of iNOS is the important part of the research toward the NO injuring brain tissue and aggravating cerebral edema.The mechanism of the NO increases after ICH ,and then induces cerebral edema may be :cerebral endothelial cell is injuried after ICH, which can produce iNOS,these iNOS can not emerge in normal, which produces the toxic NO that distribute in endothelial cell and neuron,and activate cGMP, which leads to the disequilibrium of ion distribution, in the same time ,NO accelerates the Ca2+ into cell, inhibits the energy metabolism, and then destroys brain tissue ,leads to the cerebral edema. Leucocyte and inflammatory reaction emerge in the local damage tissue ,which can activates the expression of iNOS ,so results in the expression of NO in the cerebral edema's climax. Skula A finds that the pharmacological reason of hexadecadrol inhibiting the cerebral edema is exactly the inhibition of iNOS.My experimentation finds that the expression of iNOS emerges in 48hours and 7days ,which is accordant with the expression of MMP-2,MMP-9,so we can think that cerebral edema is not produced by single reason ,but by co-effect of many reasons,so this finding can provide theoretical and experimental base for clinical curing of cerebral edema after ICH. my experimentation also finds that Angong Qingkailing can inhibits the expression of iNOS in 48hours and 7days,but Qingkailing can not inhibits the expression of iNOS,so we can regard that Angong Qingkailing can strikingly inhibits the iNOS inducing cerebral edema after ICH.3. VEGF articipates the formation of the cerebral edema after ICH Normal research thinks that VEGF can nourish much nerve cell and protect nerve system through signal pathway, ehance cell activity and survival ability and promote the axon regeneration. There are much controversy toward the function of VEGF after ICH, some research think that VEGF can express in the hematoma region after ICH ,and can react to the VEGFR-1and VEGFR-2 in the cell surface, and then activate a series of signal pathway, induce the angiogenesis, so leads to the protection of the brain tissue. other research think that VEGFcan damage BBB ,aggravate cerebral edema by itself in the early stage of ICH , combining with other factors,such as MMP-9 and Ang-2, VEGF can further aggravate cerebral edema in the middle stage of ICH.My experimentation finds that the expression of VEGF gets climax in 48hours, and can last to 7days, Angong Qingkailing can inhibits the expression of VEGF in 48hours,and can not inhibit the expression of VEGF in 7days, maybe VEGF paly the positive role in 7days.