Hydroxycamptothecin On Peripheral Lymphocyte Proliferation And Apoptosis In Rheumatoid Arthritis

ObjectiveTo investigate the effect of 10-Hydrocamptothecin(HCPT)on cell proliferation and cell apoptosis of lymphocyte cells.Furthermore,through contrast with methotrexate(MTX),try to find out the potential chloridevalue of treatment for rheumatoid arthritis(RA).Methods1.Separate and purify lymphocyte cells.Culture lymphocyte cells in vitro.2.The level of cell proliferation was measured by the MTT method.3.The level of cell apoptosis was measured by flow cytometry with Annexin v/FITC staining(FCM)method.Results1.Both HCPT and MTX can inhibit the proliferation of the cultured lymphocyte cells in vitro and the effect is dose- and time-dependent.It makes IR%increasing,which has statistic significance(p＜0.001).2.The inhibition rate of HCPT group is significant compared with MTX group(P＞0.05). The inhibition rate of HCPT group is higher than MTX group.3.Both HCPT and MTX can induce apoptosis of lymphocyte.The effect is dosedependent.4.The apoptosis ratio of lymphocyte in HCPT group is no significant differences from MTX group(P＞0.05),but the apoptosis ratio of lymphocyte in HCPT group is higher than MTX group.Conclusion1.The inhibiting capacity of HCPT on the proliferation of the cultured lymphocyte cells in vitro is superior than MTX.2.The effection of inducing apoptosis in HCPT group is to equal to which in MTX group.3.HCPT have potential value in the treatment of RA.