Hk7 Increase Du145 In Vitro Invasion And Change The Form Of Cell Growth

KLK7 is a newly discovered human kallikrein gene.hK7,the enzyme encoded by KLK7,can catalyze the degradation of intercellular cohesive structures in the cornified layer of skin and lead to desquamation.We had previously identified the expression of KLK7 in primary prostate epithelial cell and the expression can be up-regulated by the paracrine of prostate stromal cells.Given cell shedding from cancer in situ is the initiation of metastasis,we propose hypothesis that hK7 in prostate cancer tissue may facilitate the metastasis by degrading intercellular cohesive structures.To determine whether hK7 promote the malignant phenotype of prostate cancer,we transfected the human KLK7 cDNA into two prostate cancer cell line,22RV1 and DU145,which both haven't endogenous hK7 expression.Parental,vector-only transfects and KLK7 transfects subsequently subjected to MTT-based proliferation assay,in vitro Matrigel invasive assay,colony formation assay.To further confirm the effect of hK7 on the morphologic transition of DU145,conditioned medium containing high concentration of hK7 was employed to treat native DU145.Although neither the proliferation of DU145 and 22RV1 nor the colony formation percentage of DU145 alters by the expression of hK7,hK7 significantly promote the in vitro cell invasion of DU145.Furthermore,hK7 induces the epithelial-mesenchymal like transition of DU145.Our results suggested that hK7 may function as a promoter of prostate cancer metastasis.