| Depression is a common mood disorder, marked and lasting mainly depressedmood, and the corresponding change in thinking and behavior. With today's society ofthe increasingly keen competition, the increasing pace of work to accelerate, thenumber of patients with depression was significantly increased trend. Depressionserious impact on people's life, work and health. At this stage for the treatment ofdepression in Western medicine more categories, but the antidepressant effects arelimited, easy to produce resistance and apparent side effects, making Westernantidepressant was extremely restricted. Chinese medicine in the treatment ofdepression in the process, gained a wealth of theoretical knowledge and clinicalexperience. In recent years, domestic depression Chinese pathogenesis, diagnosis,prescription rule is, based on experiments conducted a more extensive and fruitfulresearch, therefore, the theory of Chinese medicine, under the guidance of use modernscientific methods study medicine antidepressant effect and mechanism, find andsecure development of antidepressant drugs is of great significance.Objective"Liver depression and phlegm" in the context of the depression formedpathogenesis, Observation Chaiqin Wendan Decoction the unpredictability of chronicmild stress model of depression (CUMS) mice, reveal the right side of depressioneffect and mechanism.Methods1 An experimental method of grouping: health Kunming 60 mice were divided intofive groups: control group (control group), model group, the positive drug controlgroup (the control group), Chaiqin Wendan Decoction low dose group (low Chai),Chaiqin Wendan Decoction high dose group (high diesel), each with 12.2 Model: Apart from the gaps in the remaining group were single cage feeding,according to the literature to establish chronic mild stress the unpredictability of depression model.3 Drug Delivery Methods: Model 18 days, Chaiqin Wendan Decoction low,highdose group of mice were given the side decoction by rly (kg·d), 20g/(kg·d)respectively, 0.1ml/10g; control group to amitriptyline 10mg (kg·d) volumesuspension gavage; blank,model transcribed equal volume saline was administeredby gavage for 18 days.4 Detection: BL-410 biological laboratory system to record the activities of the mice,5min mice independent record number of activities and record the mice within 5 minstimulation frequency. Serotonin content HPLC detection technology, plasma cortisolconcentrations were determined by radioimmunoassay.Results1 High Chai group, Chai low group and control group of mice autonomic activityincreased significantly, Compared with the control group were significantly different(P＜0.01); Chai high autonomy mice with a blank activities were not significantlydifferent (P＞0.05); Low Chai group and the control group of mice compared to HighChai group was significantly higher (P＜0.01); Low Chai group compared with thecontrol group of mice autonomic activities no significant difference (P＞0.05).2 High Chai group, Chai low group and control group of mice significantly reducedthe number of irritation, Compared with the control group were significantly different(P＜0.01); Chai group of low irritable number compared with the control group weresignificantly different (P＜0.01), Chai high group and the control group and thecontrol group were not significantly different (P＞0.05); Chai low the number ofirritation compared Chai high group and the control group were significantly different(P＜0.01); Control group with high Chai group was no significant difference (P＞0.05)3 The Model group group of 5-HT was significantly reduced compared with the blanksignificant differences (P＜0.01); High Chai group, Low Chai group and the controlgroup of 5-HT was significantly higher compared with the model group weresignificantly different (P＜0.01); High Chai group, Chai low group and control groupof 5-HT content compared with blank were not significantly different (P＞0.05);control group compared with low group and Chai Chai high group had no significant difference (P＞0.05); Low Chai group of 5-HT was significantly lower than the highChai group, compared with High Chai group significant difference (P＜0.05).4 High Chai group, Chai low group and the control group of mice plasma cortisolconcentration decreased significantly, Compared with the control group weresignificantly different (P＜0.01); High Chai group, the control group of mice plasmacortisol concentrations compared with the control group had no significant difference(P＞0.05); Low Chai group significantly lower than control group of mice plasmacortisol concentration, compared with the blank exist significant differences (P＜0.05);Chai Chai group and the high group had significantly lower (P＜0.01),Compared withthe control group are significantly different (P＜0.05); Control group with low Chaigroup was not significantly different (P＞0.05).Conclusion1 Liver depression and phlegm are depression incidence important link.2 Chaiqin Wendan Decoction effective in improving depression mice autonomyreduced, irritation and other vulnerable performance.3 Increased depression mice brain tissues of 5-HT content, lower plasma cortisolconcentrations are Chaiqin Wendan Decoction role of the Mechanism. |
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