| The purpose of this study is to examine the stability of good quality oleum fructus bruceae of freeze-dried powder, in vitro study of its release, as oleum fructus bruceae quality freeze-dried powder of the research and development to provide the basic experimental basis.oleum fructus bruceae,the oil type substances, is extracted from the hard wood species oleum fructus bruceae's ripe fruit. Modern pharmacological research shows that oleum fructus bruceae has anti-cancer, anti-pathogens, anti-oxidation, anti-ulcer effect, and so on. 10 percent of oleum fructus bruceae is made for clinical use, as a supplement anti-cancer drug for the treatment of digestive system cancer.As for oleum fructus bruceae emulsion, there is the issue of multi-phase emulsion decentralized system of unstable dynamics, heat, cold and the long-term use can be stored in milk out and caused the breakdown. Leakaged toxic oleum fructus bruceae, will seriously irritation the gastrointestinal Mucous membrane, as well as the vessel wall. In order to enhance the oleum fructus bruceae's efficacy and increase it's stability, low toxicity and side effects without affecting its anti-tumor activity, we have developed a body of oleum fructus bruceae lipsome.To enhance the stability of liposome, we used freeze-drying technology to make the freeze-dried liposome powder. The main advantage is that: the solid form of preservation, stability has been greatly improved by simply adding water and stirring. Freeze-dried liposome powder is easily and quickly to be redeveloped, while the physical and chemical properties were little changed.Adopting the ultraviolet spectrophotometry to determinate the oleum fructus bruceae content of the oleum fructus bruceae lipsomes, establish the standard curve, and the regression equation is: A = 0.4936 C + 0. 0144, r = 0. 9991. The precision and recovery of this method had complied with the request.This study used the prescription and added polymer materials to prepare blank liposomes.By studying for the encapsulation efficiency, we have determined the best prescription, that is: oleum fructus bruceae: blank liposome solution = 2.4:10, the average rate of encapsulation eddiciency is 94.32 percent. Liposomes were observed for the single-room structure, the particles are round or oval, more even distribution, and the average size is around 130 nm. Viscosity value was 4.275pa.S, pH: 6.45, Zeta potential: -23.1058 mV.Adopting the freeze-drying technology to prepare the oleum fructus bruceae freeze-dried powder of liposomes. Studying the key effects of freeze-dried process, we have determined the best preparation process, that is: pre-freezing temperature: 47℃, pre-frozen for the fast speed cooling, pre-freeze time for 4 h, vacuum drying time for 32 h. Through the microscope, rehabilitation with water, freeze-dried before and after size and distribution of the particle, encapsulation efficiency and so on as observation indicators for the screening of the types of the freeze-dried agents and determine the best prescription, that is: phospholipid and mannitol: trehalose for 1:2:2:3.Inspecting the quality of the physical and chemical properties of the freeze-dried powder, The appearance of the liposome freeze-dried powder is white and full, the average size is around140nm, pH: 6.45, Zeta potential: -23.4213 mV, encapsulation efficiency:92.22%.We have carried out the vitro release experiment of the oleum fructus bruceaa liposome freeze-dried powder. effect. Drawing the vitro release curves of the oleum fructus bruceaa. Equations were used and to study the correlation coefficient.The results showed that the oeum fructus bruceaa after being packaged by the liposome has a sustained-release... |
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