.erk / Mapk Signal Transduction Pathway In Pdgf-induced Proliferation Of Vascular Smooth Muscle Cells After Rat Abdominal Aortic Transplantation In The Role

Objective:To study the role of ERK/MAPK signal pathway in the proliferation of VSMCs induced by PDGF after abdominal aorta transplantation in ratsMethod:The chronic rejection model of abdominal aortic transplantation was established using BN rats as donors and Lewis rats as recipients.Two months later,VSMCs were isolated and cultured from allograft.The cells were identified as VSMCs by the immunohistochemical SABC method(α-SM-Actin)and passaged.The 3rd to 8th cells were used.Four groups were divided:group A:normal control;group B:PDGF-BB; group C:PD98059;group D:PDGF-BB+PD98059.48 hours later,four groups were observed by MTT to see the effect of proliferation induced by PDGF-BB and PD98059. Wstern blot assays were used to detect the expression of p21^Cip1,p27^Kip1and PCNA.The growth curve was drawn in the next four days after the cells were treated by these mediations.Result:1)in group B,cells proliferation was active,the absorbance values(A)were much higher than the other three groups,p21^Cip1,p27^Kip1were down regulated while PCNA was up-regulated.2)PD98059 can obviously block this proliferation induced by PDGF-BB. Conclusion:1)ERK/MAPK signal pathway plays an important role in the proliferation induced by PDGF in VSMCs after transplantation;2)Arteriosclerosis induced by chronic rejection could be reduced if ERK/MAPK signal pathway was blocked.